In vitro 2-photon microscope (SRS microscope)

Stimulated Raman Scattering (SRS) can occur when two photons are absorbed simultaneously. In contrast to the spontaneous Raman scattering, in this case the molecule under study is excited by the simultaneous absorption of the pump of the appropriate energy and the Stokes photon. Due to the resonant nature of the process, SRS is orders of magnitude more sensitive than spontaneous Raman scattering, but the system also has the advantages of a two-photon effect. Namely, the sample is excited only in a small volume around the focal point and an penetration depth of up to mm is available. This makes this system suitable for Ca2 + imaging and label-free cell identification too.

  • Microscope: Femto3D-AO microscope
  • Laser: Avesta Topol 1050-C (770-970 nm)