Biomedical Microbiology

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The course briefly describes the features of the human microbiota, microflora and pathogens and their interactions with the human body. It will discuss the medical/pharmaceutical role of microbial products and the impact of microbiology on the development of different industrial technologies such as pharmaceutical, medical diagnostic and food technologies. Lectures and practicals will provide an overview of the latest technologies, their theoretical background and their applicability in different industrial fields.

The practicals are aimed to familiarise the students with the basic techniques and methods of microbiology by carrying out tasks independently. Students will be familiarised with methods of sterilisation, disinfection and sanitisation, aseptic laboratory work, the use techniques of different microbiological cultures and different detection and identification methods of microbes. Genetic engineering methods used in the field of microbiology will also be introduced.

Lectures

• 12. Viral infection - Pankovics Péter
• 11. Parazites - Palkovics Tamás
• 10. Fungal pathogens - Zsoldiné Urbán Edit
• 9. Mycobacteria - Lutz Zsolt
• 8. Spirochetes, obligate intracellular pathogens - Kerényi Monika
• 7. Anerobic gram-negative bacteria - Zsoldiné Urbán Edit
• 6. Non-fermenters in human infection - Kerényi Monika
• 5. Gram- negative bacteria causing infection - Schneider György József
• 4. Anaerobic gram-pozitive bacteria causing human infections - Zsoldiné Urbán Edit
• 3. Aerobic gram-pozitive bacteria causing human infections - Kerényi Monika
• 2. Bacterial strategies (virulence factors, pathogenicity) in human body - Schneider György József
• 1. Human Microbiotas and ineraction with human body - Polgár Beáta
• 13. Microbial products in the medicine and pharmaceutical industry, vaccine developments - Polgár Beáta
• 14. Antimicrobial drugs - Zsoldiné Urbán Edit

Practices

• 14.

  Bacteriophage isolation

  - Schneider György József
• 28.

  Evaluation of the results of the molecular method

  - Pankovics Péter
• 16.

  Epidemiological investigation - plazmid preparation

  - Schneider György József
• 12.

  Sterility and pyrogenic investigation of the pharmacetical products

  - Zsoldiné Urbán Edit
• 13.

  Semi-analyser and automatisation

  - Zsoldiné Urbán Edit
• 23.

  Evaluation of ELISA test

  - Polgár Beáta
• 22.

  Serological methods in parasitology

  - Polgár Beáta
• 26.

  Experiments using molecular methods

  - Palkovics Tamás
• 25.

  Molecular methods in virology

  - Pankovics Péter
• 27.

  Perform a PCR methods

  - Pankovics Péter
• 24.

  Molecular methods in bacteriology

  - Pankovics Péter
• 7.

  Inoculation of different biochemical media

  - Tigyi Zoltán
• 21.

  Serological methods in virology diagnostic

  - Polgár Beáta
• 11.

  Evaluation of antibiotic susceptible test's results

  - Zsoldiné Urbán Edit
• 9.

  Antibiotic susceptibility tests (Disc diffusion test,)

  - Zsoldiné Urbán Edit
• 19.

  Serologic methods in the bacterial identification

  - Polgár Beáta
• 20.

  Serological methods: ELISA

  - Polgár Beáta
• 6.

  Other identification methods (e.g. MALDI-Tof MS)

  - Tigyi Zoltán
• 5.

  Identification of bacterial colonies (biochemical test)

  - Tigyi Zoltán
• 4.

  Inoculation methods from different specimens and
  culture on different media

  - Kerényi Monika
• 8.

  Evaluation of biochemical reactions

  - Tigyi Zoltán
• 15.

  Bacteriophage using as vector and control of biofilm

  - Schneider György József
• 3.

  Staining methods Gram's staining, simple staining, acid-fast staining, capsule staining

  - Kerényi Monika
• 2.

  Microbiological methods in diagnostic laboratory, preparation and staining of smear , microscopy

  - Kerényi Monika
• 10.

  Antibiotic sensitivity tests -microdilutions, and macrodilution and agar dilution methods

  - Zsoldiné Urbán Edit
• 1.

  Preparation of different liquid and solid media

  - Kerényi Monika
• 17.

  Perform an experiment

  - Schneider György József
• 18.

  Evaluation of the microbiological experiments

  - Schneider György József

Seminars

Reading material

Obligatory literature

Ford, Michael: Medical Microbiology (Fundamentals of Biomedical Science), ISBN-13: 978-0198818144, ISBN-10: 0198818149

Lee Yuan Kun ed. Microbial Biotechnology: Principles and Applications (3rd Edition ) 2013 ISBN-13: 978-9814366823 ISBN-10: 981436682X

Literature developed by the Department

lecture notes on the teams

Notes

Recommended literature

Madigan, Michael T.; Bender, Kelly S.; Buckley, Daniel H.; Sattley, W. Matthew; Stahl, David A.: Brock Biology of Microorganisms (16th Edition) 2020, Person ISBN 10: 0134874404 ISBN 13:9780134874401

Conditions for acceptance of the semester

Preparing and submitting the protocols and evaluation of results of the experiments and tests carried out during the practicals to the supervisors.

Mid-term exams

Completed assessment in laboratory practice is required

Making up for missed classes

Consultation with the supervisor

Exam topics/questions

Topics of the laboratory practices

Examiners

• Kerényi Monika
• Pankovics Péter
• Polgár Beáta
• Schneider György József
• Tigyi Zoltán
• Zsoldiné Urbán Edit

Instructor / tutor of practices and seminars

• Kerényi Monika
• Kocsis Béla
• Lutz Zsolt
• Palkovics Tamás
• Pankovics Péter
• Polgár Beáta
• Schneider György József
• Tigyi Zoltán
• Zsoldiné Urbán Edit