Microbiology 2 - Practice

Data

Official data in SubjectManager for the following academic year: 2025-2026

Topic

The objective is to provide a solid knowledge of microbiological diagnostic methods, sterility and pyrogenicity tests. By active participation in the practical, students will gain the skill of aseptic technique in microbiology and pharmacology.

Lectures

Practices

• 1.

  Introduction, safety regulations. The microscope, wet mount and stained preparations

  - Tigyi Zoltán
• 2.

  Introduction, safety regulations. The microscope, wet mount and stained preparations

  - Tigyi Zoltán
• 3.

  Cultivation of bacteria, media and techniques.

  - Melegh Szilvia Zsóka
• 4.

  Cultivation of bacteria, media and techniques.

  - Melegh Szilvia Zsóka
• 5. Identification of bacteria - Tigyi Zoltán
• 6. Identification of bacteria - Tigyi Zoltán
• 7.

  Serological methods in microbiology. Immunotherapy.

  - Zsoldiné Urbán Edit
• 8.

  Serological methods in microbiology. Immunotherapy.

  - Zsoldiné Urbán Edit
• 9.

  Molecular methods in clinical microbiology

  - Polgár Beáta
• 10.

  Molecular methods in clinical microbiology

  - Polgár Beáta
• 11. Sterilisation and disinfection, Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora - Kocsis Béla
• 12. Sterilisation and disinfection, Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora - Kocsis Béla
• 13. Antibiotic sensitivity (Kirby-Bauer method, MIC, MBC, E-test) - Zsoldiné Urbán Edit
• 14. Antibiotic sensitivity (Kirby-Bauer method, MIC, MBC, E-test) - Zsoldiné Urbán Edit
• 15. Antibiotic concentration in body fluids. Detection of enzymes and compounds which neutralize antibiotics. Interactions of antibiotics - Mestyán Gyula
• 16. Antibiotic concentration in body fluids. Detection of enzymes and compounds which neutralize antibiotics. Interactions of antibiotics - Mestyán Gyula
• 17. Diagnosis of bacterial wound infection. Sepsis - blood culture - Zsoldiné Urbán Edit
• 18. Diagnosis of bacterial wound infection. Sepsis - blood culture - Zsoldiné Urbán Edit
• 19. Bacterial diagnosis of gastrointestinal infections - Tigyi Zoltán
• 20. Bacterial diagnosis of gastrointestinal infections - Tigyi Zoltán
• 21.

  Bacterial diagnosis of urinary tract infections

  - Melegh Szilvia Zsóka
• 22.

  Bacterial diagnosis of urinary tract infections

  - Melegh Szilvia Zsóka
• 23. Bacterial diagnosis of respiratory tract infections - Mestyán Gyula
• 24. Bacterial diagnosis of respiratory tract infections - Mestyán Gyula
• 25. Microbiological diagnosis of fungal and parasitic infections - Mestyán Gyula
• 26. Microbiological diagnosis of fungal and parasitic infections - Mestyán Gyula
• 27.

  Methods used in diagnostic virology

  - Boros Ákos
• 28.

  Methods used in diagnostic virology

  - Boros Ákos

Seminars

Reading material

Obligatory literature

Denyer SP ,Hodges NA, Gorman SP, Gilmore BF: Hugo and Russells Pharmaceutical Microbiology, 8th Edition, Blackwell Science Ltd

2018. ISBN: 978-1-119-43449-8

Tim Sandle: Pharmaceutical Microbiology : Essentials for Quality Assurance and Quality Control, ELSEVIER SCIENCE & TECHNOLOGY ,

Woodhead Publishing Ltd, 2015 ISBN10 0081000227

Literature developed by the Department

Practical materials on the Teams

Notes

Practical materials on the Teams

Recommended literature

Dr Patrick R. Murray, Dr Ken S. Rosenthal and Dr Michael A. Pfaller (eds.): Medical Microbiology, 9th edition, Elsevier Saunders 2020 ISBN13 (EAN): 9780323673228

Conditions for acceptance of the semester

-

Mid-term exams

End of the course the students will take a lab practice exam.

Making up for missed classes

Consultation with supervisor

Exam topics/questions

Exam questions are based on the topic of the practicals.

Examiners

Instructor / tutor of practices and seminars

• Boros Ákos
• Kocsis Béla
• Melegh Szilvia Zsóka
• Mestyán Gyula
• Polgár Beáta
• Tigyi Zoltán
• Zsoldiné Urbán Edit