Molecular Cell Biology 1

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

Molecular cell biology aims to introduce the molecular mechanisms of molecular biology and cell biology and to emphasise their importance in medicine. It provides the basis for teaching the different areas of molecular medicine, molecular pathology, molecular diagnostics, pharmacology and gene therapy. The first semester of the course will introduce the molecular biology methods used today, the structure and function of cellular components, the regulation of the cell cycle, the mechanisms of DNA replication and the expression of gene expression.

Lectures

• 1. Educational objectives - Pap Marianna
• 2. Comparison of prokaryotic and eukaryotic cells - Kemény Ágnes
• 3. Macromolecules I. Nucleic acids - Pap Marianna
• 4. Macromolecules II. Proteins - Pap Marianna
• 5.

  Modern morphological methods I. Light micrsocopy

  - Bugyi Beáta
• 6.

  Modern morphological methods II. Electronmicrsocopy

  - Bugyi Beáta
• 7. Molecular biology methods I. Restriction endonucleases - Pap Marianna
• 8. Molecular biology methods II. DNA cloning. Genomic libraries - Pap Marianna
• 9. Molecular biology methods III. Polymerase chain reaction - Pap Marianna
• 10. Molecular biology methods IV. DNA sequencing: Sanger-sequencing, DNA-chip - Pap Marianna
• 11. Molecular biology methods V. DNA sequencing: New generation sequencing - Pap Marianna
• 12. Molecular biology methods VI. Analysis of gene expression - Pap Marianna
• 13. Molecular biology methods VII. Transgenic organisms - Pap Marianna
• 14. Molecular biology methods VIII. Inhibition of gene expression - Pap Marianna
• 15. Molecular biology methods IX. Immunologioal methods - Pap Marianna
• 16.

  The cell nucleus

  - Bátor Judit
• 17. Genome organization - Kemény Ágnes
• 18.

  The structure and chemical composition of chromatin

  - Varga Judit
• 19. Phases of the cell cycle - Sétáló György ifj.
• 20. Regulation of the cell cycle - Sétáló György ifj.
• 21. Cell division. Mitosis, meiosis - Sétáló György ifj.
• 22. DNA replication in prokaryotes - Sétáló György ifj.
• 23. DNA replication in eukaryotes - Sétáló György ifj.
• 24. DNA repair - Sétáló György ifj.
• 25. Transcription I. Transcription in prokaryotes - Pap Marianna
• 26. Transcription II. Synthesis and processing of pre-rRNA in eukaryotes - Pap Marianna
• 27. Transcription III. Synthesis of pre-mRNA in eukaryotes - Pap Marianna
• 28. Transcription IV. pre-m RNA processing in eukaryotes - Pap Marianna
• 29. Translation I. The components of protein synthesis - Szeberényi József
• 30. Translation II. The mechanism of protein synthesis - Szeberényi József
• 31. Translation III. The genetic code - Szeberényi József
• 32. Regulation of gene expression in prokaryotes - Pap Marianna
• 33. Regulation of gene expression in eukaryotes I: Levels of gene regulation - Szeberényi József
• 34. Regulation of gene expression in eukaryotes II: Epigenetics - Szeberényi József
• 35. Regulation of gene expression in eukaryotes III: Transcription factors - Szeberényi József
• 36. Rough endoplasmic reticulum. Golgi-aaparatus. Protein glycosylation - Kemény Ágnes
• 37. Endocytosis. Mechanism of vesicular transport - Kemény Ágnes
• 38. Cell defense mechanisms I. Lysosomes, smooth endoplasmic reticulum - Kemény Ágnes
• 39. Cell defense mechanisms II. Biological effict od oxygen free radicals - Kemény Ágnes
• 40. Mitochondria I. Structure and function - Sétáló György ifj.
• 41. Mitochondria II. Genetic apparatus - Sétáló György ifj.
• 42. Closing lecture. Exam rules - Pap Marianna

Practices

• 1. Light microscopy. Tracing biological molecules inside the cell
• 2. Light microscopy. Tracing of biological molecules inside the cell
• 3. Polarization and phase contrast microscopy
• 4. Polarization and phase contrast microscopy
• 5. Centrifugation, chromatography
• 6. Centrifugation, chromatography
• 7. Plasmid isolation
• 8. Plasmid isolation
• 9. SDS polyacrylamide gel electrophoresis and Westerm blotting
• 10. SDS polyacrylamide gel electrophoresis and Westerm blotting
• 11. Isolation of nucleic acids
• 12. Isolation of nucleic acids

Seminars

• 1. General information
• 2. Biological macromolecules
• 3. Separation methods
• 4. Comparison of pro- and eukaryotic cells
• 5. Molecular biology methods I.
• 6. Molecular biology methods II.
• 7. Molecular biology methods III.
• 8. Molecular biology methods IV.
• 9. Cell nucleus
• 10. Genome organization
• 11. Chromatin
• 12. Cell cycle
• 13. Regulation of cell cycle
• 14. Cell division. Mitosis, meiosis
• 15. DNA replication
• 16. DNA repair
• 17. Transcription in prokaryotes
• 18. Transcription in eukaryotes
• 19. RNA processing
• 20. Translation I.
• 21. Translation II.
• 22. Regulation of gene expression I.
• 23. Regulation of gene expresssion II.
• 24. Regulation of gene expresssion III.
• 25. Rough endoplasmic reticulum. Golgi complex
• 26. Vesicular transport
• 27. Cell defense mechanisms
• 28. Mitochondria
• 29. Final test
• 30. Final test

Reading material

Obligatory literature

Cooper, G.M.: The Cell. A Molecular Approach.

Literature developed by the Department

Materials uploaded to Potepedia

Test bank questions

Minimum requirement exam questions

Notes

M. Pap (editor): Molecular Cell Biology Laboratory Manual

Szeberényi J., Komáromy L.: Molecular Cell Biology Syllabus

Recommended literature

Conditions for acceptance of the semester

None

Mid-term exams

Midterm tests in weeks 4, 7 and 10, from lecture and seminar topics. Test topics will be announced in lectures and in the Molecular Cell Biology 1 Lecture Teams group. The final test is in week 14, from the whole semester topics (lectures, seminars and lab materials are included). No improvement is possible on any of the written tests.

Making up for missed classes

It is possible to make up the lab if a medical certificate is submitted, either in another group or in a make-up lab organized by the Medical Biology Department.
Midterm tests can be made up by oral exam if a medical certificate is submitted.

There is no possibility to make up the final semester test.

Exam topics/questions

1.      Comparison of prokaryotic and eukaryotic cells

2.      Nucleosides, nucleotides

3.      Structure and properties of DNA

4.      Experiments demonstrating the role of DNA as genetic material

5.      Structure and types of RNA

6.      Structure and properties of proteins

7.      Types of carbohydrates and their biological significance

8.      Types of lipids and their biological significance

9.      Characteristics of restriction endonucleases and their applications

10.  Steps of DNA fragment cloning

11.  Genomic library construction and screening

12.  Steps and types of the polymerase chain reaction and their applications

13.  Steps and types of Sanger DNA sequencing

14.  Steps of Illumina DNA sequencing

15.  Steps of proton sequencing and nanopore sequencing

16.  Types of DNA chips and their applications

17.  Types of fluorescence in situ hybridization and their applications

18.  Biological significance of the Human Genome Project

19.  Steps of cDNA cloning

20.  cDNA library construction and screening

21.  Generation of transgenic organisms and their practical significance

22.  Inhibition of endogenous gene expression at the DNA level

23.  Inhibition of endogenous gene expression at the mRNA level

24.  Inhibition of endogenous gene expression at the protein level

25.  Methods of specific antigens’ microscopic detection

26.  Steps of immunoprecipitation and Western blotting

27.  Structure of the nucleus

28.  Types of repetitive and unique sequences and their medical significance

29.  Levels of chromatin organization

30.  Chemical composition of chromatin

31.  Phases of the cell cycle

32.  Regulation of the cell cycle

33.  Mitosis

34.  Meiosis

35.  General features of replication

36.  Mechanism of prokaryotic replication

37.  Specific features of eukaryotic replication

38.  DNA repair - Types of excision repairs and their medical significance

39.  DNA repair - Proofreading, Mismatch repair and their medical significance

40.  DNA repair - Types of DNA double-strand breaks’ repairs and their medical significance

41.  Mechanism of prokaryotic transcription and RNA processing

42.  General features of eukaryotic transcription

43.  Eukaryotic pre-rRNA synthesis and processing

44.  Eukaryotic pre-mRNA synthesis and processing. Cap formation and polyadenylation

45.  Pre-mRNA splicing, RNA editing and their medical significance

46.  Steps of aminoacyl-tRNA synthesis

47.  Structure and function of ribosomes

48.  Main features of the genetic code

49.  Initiation of translation

50.  Elongation and termination of translation

51.  General features of translation, and the medical significance of its inhibition

52.  The lactose operon

53.  The tryptophan operon

54.  Cloning by cell nuclear transplantation and its medical significance

55.  Regulation of pre-mRNA synthesis and processing in eukaryotes

56.  Regulation of mRNA transport, translation and degradation in eukaryotes

57.  Regulation of protein activity and degradation in eukaryotes

58.  Types of eukaryotic transcription factors

59.  Mechanism of action of steroid hormones and its medical significance

60.  Function of the rough endoplasmic reticulum

61.  Function of the Golgi apparatus. Types of protein glycosylation and their medical significance

62.  Mechanism and types of secretion

63.  Types of endocytosis and their medical significance

64.  Mechanism of vesicular transport

65.  Types of lysosomes and their medical significance

66.  Function of the smooth endoplasmic reticulum

67.  Generation of oxygen free radicals and their biological effects

68.  Structure and function of mitochondria

69.  Genetic apparatus of mitochondria

70.  Types of mitochondrial diseases

Examiners

• Atlaszné Váczy Alexandra
• Balassa Tímea
• Balogh Bálint
• Bátor Judit
• Berta Gergely
• Bogdán Ágnes
• Boros Melinda
• Csabai-Tanics Tímea Judith
• Horváth Marianna
• Kemény Ágnes
• Németh Marica
• Pap Marianna
• Sétáló György ifj.
• Tarjányi Oktávia
• Varga Judit

Instructor / tutor of practices and seminars

• Atlaszné Váczy Alexandra
• Balassa Tímea
• Balogh Bálint
• Bátor Judit
• Berta Gergely
• Bogdán Ágnes
• Boros Melinda
• Bugyi Beáta
• Csabai-Tanics Tímea Judith
• Feketéné Kiss Katalin
• Horváth Marianna
• Kemény Ágnes
• KURZUSHOZ RENDELT OKTATÓ
• Németh Marica
• Pap Marianna
• Sétáló György ifj.
• Tarjányi Oktávia
• Varga Judit