Molecular Cell Biology for Students of Dentistry 1

Data

Official data in SubjectManager for the following academic year: 2019-2020

Topic

To provide a molecular and cell biological basis for anatomy, biochemistry, physiology, pathology, pathophysiology, microbiology and pharmacology studies of dentistry students. The course covers cellular and molecular characteristics of the structure and function of cells, the mechanisms of storage, replication and expression of the genetic information and the methods used to study them.

Lectures

• 1. Introduction - Dr. Sétáló György
• 2. Cellular organization in general - Dr. Sétáló György
• 3. Nucleic acids - Dr. Sétáló György
• 4. Proteins - Dr. Sétáló György
• 5. Lipids and carbohydrates - Dr. Sétáló György
• 6. Light microscopy - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 7. Electron microscopy - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 8. Restriction endonucleases and modification methylases - Dr. Sétáló György
• 9. DNA amplification in bacterial systems - Dr. Sétáló György
• 10. DNA amplification in vitro - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 11. The Sanger method - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 12. Genetically modified organisms - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 13. DNA chips - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 14. The cell nucleus - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 15. Unique and repetitive sequences - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 16. The structure and chemical composition of chromatin - Dr. Sétáló György
• 17. Interphase, mitosis and meiosis - Dr. Sétáló György
• 18. Regulation of the cell cycle - Dr. Sétáló György
• 19. DNA synthesis in prokaryotes - Dr. Sétáló György
• 20. DNA synthesis in eukaryotes - Dr. Sétáló György
• 21. DNA repair - Dr. Sétáló György
• 22. RNA synthesis in prokaryotes - Dr. Pap Marianna
• 23. rRNA synthesis in eukaryotes - Dr. Pap Marianna
• 24. mRNA transcription in eukaryotes, modifications of the 5’ and 3’ ends - Dr. Pap Marianna
• 25. Processing of pre-m RNA in eukaryotes - Dr. Pap Marianna
• 26. Cytoplasmic organelles - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 27. The components of protein synthesis - Dr. Pap Marianna
• 28. The genetic code - Dr. Pap Marianna
• 29. The mechanism of translation - Dr. Pap Marianna
• 30. Regulation of gene expression in prokaryotes - Feketéné Dr. Kiss Katalin
• 31. Levels of gene regulation in eukaryotes - Dr. Pap Marianna
• 32. Transcription factors - Dr. Pap Marianna
• 33. Rough endoplasmic reticulum - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 34. Golgi complex, secretion - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 35. Endocytosis, vesicular transport - Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• 36. Lysosomes, smooth endoplasmic reticulum - Dr. Bátor Judit
• 37. Oxygen free radicals, membrane damage - Dr. Kemény Ágnes
• 38. ATP synthesis in mitochondria - Dr. Sétáló György
• 39. Mitochondrial DNA - Dr. Sétáló György
• 40. Microfilaments - Dr. Sétáló György
• 41. Intermediate filaments and microtubules - Dr. Fekete Zsuzsanna
• 42. The cell membrane - Dr. Berta Gergely

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Szeberényi J., Komáromy L.: Molecular Cell Biology Syllabus.

Notes

Recommended literature

Gerald Karp: Cell and Molecular Biology
Cooper, G.M.: The Cell. A Molecular Approach.
Lodish et al.: Molecular Cell Biology
Alberts et al.: Molecular Biology of the Cell
Szeberényi J.: Experiments in Molecular Cell Biology

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

Mid-term tests

Making up for missed classes

It is not possible to make up for missed lectures.

Exam topics/questions

1. Proteins
2. Lipids
3. Carbohydrates
4. Nucleosides, nucleotides
5. The DNA molecule
6. DNA as the genetic material (experimental evidences)
7. Types of RNA
8. Pro- and eukaryotic cells
9. Methods of immunocytochemistry
10. Restriction endonucleases
11. Southern blotting
12. Determining the sequence of DNA
13. DNA chips
14. Genomic libraries
15. Polymerase chain reaction
16. Transgenic organisms
17. Targeted gene inactivation at the DNA level
18. Inhibition of gene expression at the level of mRNA
19. cDNA libraries
20. Northern blotting
21. Immunoprecipitation and Western blotting
22. The structure of the nucleus
23. Multilevel chromatin organization
24. Unique and repetitive sequences
25. The chemical composition of chromatin
26. Phases of the cell cycle
27. Regulation of the cell cycle
28. Mitosis
29. Meiosis
30. General features of replication
31. Mechanism of replication in prokaryotes
32. Eukaryotic replication
33. DNA repair
34. The mechanism of prokaryotic transcription
35. General features of eukaryotic transcription
36. Synthesis and processing of eukaryotic pre-rRNA
37. Synthesis of pre-mRNA in eukaryotes, modifications at the 5’ and 3’ ends
38. Pre-mRNA splicing
39. Synthesis of aminoacyl-tRNA
40. The structure and function of ribosomes
41. The genetic code
42. Initiation of translation
43. Elongation and termination of translation
44. General features of translation
45. The lactose operon
46. The tryptophan operon
47. Cloning by nuclear transplantation
48. Regulation of pre-mRNA synthesis and processing in eukaryotes
49. Regulation of mRNA transport, translation and degradation in eukaryotes
50. Regulation of protein activity and degradation in eukaryotes
51. Eukaryotic transcription factors
52. The mechanism of action of steroid hormones
53. Rough endoplasmic reticulum
54. Golgi complex and the glycosylation of proteins
55. The mechanism of secretion
56. Endocytosis
57. The mechanism of vesicular transport
58. Lysosomes and the smooth endoplasmic reticulum
59. Oxygen free radicals and membrane damage
60. The structure and function of mitochondria
61. The genetic apparatus of mitochondria
62. Mitochondrial diseases

Examiners

• Balogh Bálint
• Csabai-Tanics Tímea Judith
• Dr. Bátor Judit
• Dr. Berta Gergely
• Dr. Czéh Boldizsár
• Dr. Fekete Zsuzsanna
• Dr. Kemény Ágnes
• Dr. Pap Marianna
• Dr. Rimayné Dr. Ábrahám Hajnalka Gabriella
• Dr. Sétáló György
• Dr. Tarjányi Oktávia
• Feketéné Dr. Kiss Katalin
• Les Hajnalka
• Németh Marica
• Schipp Renáta
• Varga Judit

Instructor / tutor of practices and seminars