Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Pap Marianna
associate professor,
Department of Medical Biology and Central Electron Microscope Laboratory -
Number of hours/semester
lectures: 24 hours
practices: 0 hours
seminars: 0 hours
total of: 24 hours
Subject data
- Code of subject: OSF-MMO-T
- 2 kredit
- Dentistry
- Optional modul
- spring
OSA-MF1-T finished
Course headcount limitations
min. 5 – max. 200
Topic
The aim of the course is to describe interesting and novel molecular biology technologies, including three-dimensional tissue culturing, bioprinting, new generation sequencing techniques, human genetic markers, targeted genome editing. We emphasize the importance and applicability of these methods in research, in the modeling and diagnosis of different diseases.
Lectures
- 1. Types of cell cultures - Pap Marianna
- 2. Maintenance and manipulation of cell cultures - Pap Marianna
- 3. 3D tissue culturing - general features - Pap Marianna
- 4. 3D tissue culturing - cells and biomaterials - Pap Marianna
- 5. Bioprinting - general features - Pap Marianna
- 6. Bioprinting - medical application - Pap Marianna
- 7. Types of PCR - Pap Marianna
- 8. PCR - medical application - Pap Marianna
- 9. Next generation sequencing techniques - Pap Marianna
- 10. Next generation sequencing techniques - Pap Marianna
- 11. Human genetic markers - general features - Pap Marianna
- 12. Methods to study human genetic markers - Pap Marianna
- 13. Genome editing - sequence-specific nucleases - Pap Marianna
- 14. Genome editing - CRISPR/Cas9 system and medical application - Pap Marianna
- 15. Epigenetics - Pap Marianna
- 16. Methods to study DNA methylation - Pap Marianna
- 17. Functional genomics - methods to study transcription - Pap Marianna
- 18. Functional genomics - methods to study translation - Pap Marianna
- 19. Molecular diagnostics - Pap Marianna
- 20. Molecular diagnostics - Pap Marianna
- 21. Types of gene therapy - Pap Marianna
- 22. Gene therapy - medical application - Pap Marianna
- 23. Exam - Pap Marianna
- 24. Exam - Pap Marianna
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Lecture slides
Notes
Recommended literature
Conditions for acceptance of the semester
None
Mid-term exams
Written test on the last week.
Making up for missed classes
No possibility to make up the missing lectures.
Exam topics/questions
1. Types of cell cultures
2. Maintenance and manipulation of cell cultures
3. 3D tissue culturing, Bioprinting
4. Types of PCR
5. Next generation sequencing techniques
6. Human genetic markers
7. Genome editing
8. Epigenetics
9. Functional genomics
10. Molecular diagnostics
11. Gene therapy, medical application