Supervisor: ÁBRAHÁM, Hajnalka
Co-supervisor: Prof. Dr. SERESS, László
The aim of the study is to find the changes that could be seen in the cerebral cortex in epilepsy. The samples used for the histological examinations are from patients, who were operated in the Dept. of Neurosurgery because of epilepsy. In the last years, we have found interesting data, namely, the presence of large numbers of neurons in the white matter. Our aim is to study the role of these cells. We use immunocitochemistry, confocal laser scanning microscopy and electron microscopy to characterize these neurons. Moreover, computerized methods will be used to count these cells. Data obtained will be correlated with the clinical and general histological data of the patients.
The tasks of the student(s) who is/are interested in it, will the computerized cell counting, the examinations of these neurons with light microscope and they will do the correlation between histological and clinical data. Moreover, they will learn the histological methods used for the study.
Students with interest towards histology, pathology, neurology after the neuroanatomy and physiology final exams are welcome.
Total joint replacement (TJR) has allowed many end-stage arthritis patients to improve their quality of life. However, 15-20% of TJRs fail and require revision arthroplasty, and about 70% of these surgeries are performed because of implant loosening, which is attributed to resorption of bone tissue around the implant (periprosthetic osteolysis [PPO]). Key players of PPO pathogenesis are wear particles of the implants, which are internalized by macrophages and promote pro-inflammatory cytokine expression followed by osteoclastogenesis and bone resorption. Some patients are particularly sensitive to PPO, which might be explained by the implication of certain genes and epigenetic factors. Students will investigate the genetic and epigenetic aspects of PPO by exploring the involved genes, disease-related epigenetic profiles and polymorphisms. Students will learn state-of-the-art molecular techniques, bioinformatics and statistical methodologies for exploring the pathogenesis-involved factors. Dedicated students are interested in both clinical and molecular aspects of PPO are very welcomed in our lab.
Supervisor: SZEBERÉNYI, József
Co-supervisor: FEKETÉNÉ DR. KISS, Katalin
The phaeochromocytoma cell lines (PC12 cells) stop proliferating, enter G0 phase and differentiate into neuronal cells upon nerve growth factor (NGF) treatment. Beside these effects NGF promotes cell survival as well, the withdrawal of NGF from the cells (in the lack of other growth factors) induces apoptosis. Based on these biological effects the PC12 cells serve as a really good model to study the signal transductions of neuronal differentiation, cellular quiescence and apoptosis or cell survival.
Our research work focuses on two, partly communicating fields: we study the intracellular signaling pathways of NGF resulting in the antiproliferative and the antiapoptotic effects. The central role of Ras and RhoA proteins (members of Ras/monomer G-proteins superfamily) is well known in these processes. We study their downstream signaling connections, especially their effects on the activation of PI3K enzyme and NFkB transcription factor.
The member of the research team: Ibolya Koloszár
Supervisor: PAP, Marianna
The signaling of MAP-Kinases is studied in wild type and mutant rat pheochromocytoma (PC12) cell cultures. These enzymes are known mediators of neuronal differentiation and various stress inducers. Currently we are studying the effects of agents from the family of proteasome inhibitors (like MG-132 and epoxomicin) that can elicit neuronal differentiation in this cell type. Other members of the group are studying signaling mechanisms initiated by the peptide urocortin 2 in PC12 cells. From these observations the ability of this peptide ligand to modulate the action of nerve growth factor (NGF) appear to be especially interesting. The members of the research team: Alexandra Harci, OktáviaTarjányi MD, Mónika Vecsernyés. We work in cooperation with Dr. Tamás Atlasz at Faculty of Sciences, UP
Co-supervisor: Prof. Dr. SZEBERÉNYI, József
Nitric oxide (NO), as a second messenger, plays multiple roles in intracellular signal transduction events. The signaling pathways leading to survival or apoptosis of different cell types are mostly well known. Nitric oxide, by activating or inactivating proteins involved in the above mentioned processes, plays a modulatory role in the regulation of these signaling pathways. Melanoma cell lines treated with high (e.g. toxic) doses of sodium nitroprusside (SNP), a molecule which forms nitric oxide are used in our experiments. Our task is to identify changes in activation or expression of proteins involved in apoptosis.
The periodontal ligament (PDL) plays an important role in the changes of the periodontal connective and osseus tissue caused by physiological forces and by pressure applied during orthodontic treatment. The aim of our experiments is to study the effects of continuous mechanical stress of physiological magnitude on the level of PDL derived cell
cultures, and also to examine the signalling events behind these effects. The members of the research tem: Dr. József Szeberényi professor, Dr. György Sétáló jr., Mónika Vecsernyés, Kata Janovics
Our research team focuses on the role of the CREB transcription factor in the survival, programmed cell death and neuronal differentiation of cell cultures. The CREB transcription factor regulates the transcription of at least 2000 genes of the human genome (approximately 10% of the total number of genes). Its role has been proven in learning, in several neurodegenerative diseases and in the survival of neurons. The research covers the following two areas of interest.
Investigation of alcohol-stress
The proper mechanism of alcoholism and alcohol-stress has not been known in details. The aim of the research is to identify and characterize the alcohol-induced signaling pathways, to explore the relationship between CREB and alcoholism.
Exploration of endoplasmic reticulum stress
Cells can undergo programmed cell death p53-independently due to extensive endoplasmic reticulum stress. Endoplasmic reticulum stress has a role in the development of several diseases, and its selective induction might be a promising target of the therapy of p53-negative tumors. The experiments investigate the role of the CREB protein in endoplasmic reticulum stress. The members of the research team: András Balogh MD, Ibolya Koloszár, Kazushi Jinno, Mária Németh, Csilla Szigeti. We work in cooperation with: Max-Delbrück Center, Berlin and School of Medicine Osijek.
Supervisor: SZEBERÉNYI, József
Nitric oxide (NO), as a second messenger, plays multiple roles in intra- and intercellular signal transduction events. The signaling pathways leading to apoptosis, proliferation or differentiation of PC12 cells are well known. Nitric oxide, by activating or inactivating proteins involved in the above mentioned processes, plays a paramount role in the regulation of these signaling pathways. Our research team explores the role played by the members of the Ras family and p53 protein in these events.
Interferons are involved in the antiviral response of many cell types. The effects of interferon-alpha are also investigated in PC12 cells (later possibly also in other cell types).
A kutatócsoport tagjai: Judit Bátor MD, Szilvia Galgóci PD, Gergő Nádasdi, Zita Potzné Árvai, Judit Varga
Co-supervisor: SCHIPP, Renáta
PKR is a serine/threonin specific kinase, which has an important role in the mediation of interferon induced antiviral response, in the regulation of cell proliferation, differentiation. Trough the activation of different transcription factors (STAT, p53, NFκB) and the phosphorylation of the eukaryotic initiation factor 2 alpha (eIF2α) PKR has a key role in the regulation of apoptosis too. Signal transduction effects of PKR are being studied using clones expressing PKR siRNA. The members of the research team: Renáta Hargitai, Zita Potzné Árvai, Mónika Vecsernyés .