Advanced Tissue Culture Technologies - Lecture

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The course material is designed to provide theoretical background to currently employed three dimensional tissue culture techniques including scaffold-based (biodegradable) and scaffold-free (tissue printing, pelleting and hanging-drop) systems. The course will detail the importance of the tissue microenvironment in normal and artificial tissue development. Directed cellular growth, organ formation for surgical implantation and drug target identification in human tissue systems and in vitro testing of pharmaceutical compounds in complex tissue systems are also part of the curriculum. The course will discuss the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions.

Lectures

• 1. Introduction, basic principles of tissue engineering (TE) - Bóvári-Biri Judit
• 2. 2D and 3D cell and tissue culture - Bóvári-Biri Judit
• 3. Cells and tissue types in tissue engineering I: stem cell types, their characteristics and potential applications (hESCs, iPSCs, fetal stem cells) - Bóvári-Biri Judit
• 4. Cells and tissue types in tissue engineering II: stem cell types, their characteristics and potential applications (adult somatic stem cells, eg. ADSCs, dental stem cells). Differentiated, organ-specific primary cells. - Bóvári-Biri Judit
• 5. Bioreactors, requirements of bioreactor design. Characteristics and use of the main bioreactor types I. - Bóvári-Biri Judit
• 6. Characteristics and use of the main bioreactor types II. - Bóvári-Biri Judit
• 7. Scaffolds in tissue engineering. Types and characteristics of frequently used biomaterials I. - Bóvári-Biri Judit
• 8. Types and characteristics of frequently used biomaterials II. - Bóvári-Biri Judit
• 9. Degradation of polymer scaffolds. Technologies of scaffold fabrication I. - Bóvári-Biri Judit
• 10. Technologies of scaffold fabrication II. - Bóvári-Biri Judit
• 11. Cell-scaffold interactions. Scaffold parameters affecting cell behavior. - Bóvári-Biri Judit
• 12. Biofactors in TE. Controlled release of bioactive factors in engineered tissues. Drug delivery systems in TE I. - Bóvári-Biri Judit
• 13. Drug delivery systems in TE II. Biosensors in TE. - Bóvári-Biri Judit
• 14. Types and use of aggregate cultures. Organoids and spheroid cultures. - Bóvári-Biri Judit
• 15. Technologies used for the generation of aggregate cultures I. - Bóvári-Biri Judit
• 16. Technologies used for the generation of aggregate cultures II. Microfluidic systems. - Bóvári-Biri Judit
• 17. Advanced tissue models using transwell inserts: Air-Liquid Interface (ALI) technology and Blood-Brain-Barrier (BBB) models. - Bóvári-Biri Judit
• 18. Test - Bóvári-Biri Judit
• 19. Tumor models I. - Bóvári-Biri Judit
• 20. Tumor models II. - Bóvári-Biri Judit
• 21. Tissue printing technologies I. - Bóvári-Biri Judit
• 22. Tissue printing technologies II. - Bóvári-Biri Judit
• 23. Tissue engineering for transplantation I. Timeline and milestones of organ and tissue transplantation - Bóvári-Biri Judit
• 24. Hematopoietic stem cell transplantation. - Bóvári-Biri Judit
• 25. Tissue engineering for transplantation I. TE of pancreatic islets - Bóvári-Biri Judit
• 26. Tissue engineering for transplantation II. Cartilage TE. - Bóvári-Biri Judit
• 27. Tissue engineering for transplantation II. Skin substitutes. - Bóvári-Biri Judit
• 28. Types and regulation of advanced therapy medicinal products (ATMPs). - Bóvári-Biri Judit

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Lecture ppt

Notes

Three dimensional tissue cultures and tissue engineering -access via www.gytk.hu

Three dimensional tissue cultures and tissue engineering - access via www.tankonyvtar.hu

Recommended literature

Pongracz & Keen: Medical Biotechnology, Elsevier, 2009

Joseph D. Bronzino: Tissue Engineering and Artificial Organs

Ali Khademhosseini (ed.): Micro- and Nanoengineering of the Cell Microenvironment, Technologies and Applications (Engineering in Medicine & Biology)

Challa S. S. R. Kumar (ed.): Tissue, Cell and Organ Engineering (Nanotechnologies for the Life Sciences)

W. Mark Saltzman: Tissue Engineering: Engineering Principles for the Design of Replacement Organs and Tissues

Robert Lanza, Robert Langer, Joseph Vacanti: Principles of Tissue Engineering, 3rd edition

Will W. Minuth, Raimund Strehl, Karl Schumacher: Tissue Engineering: From Cell Biology to Artificial Organs

Fen Ontaigne: Medicine by Design: The Practice and Promise of Biomedical Engineering, Forgacs & Newman, 2005.

Conditions for acceptance of the semester

75% attendance to lectures and completion of the midterm test, then final exam

Mid-term exams

Midterm test

Making up for missed classes

None

Exam topics/questions

Written test. Online Unipol system, Teams, Moodle

Examiners

• Bóvári-Biri Judit

Instructor / tutor of practices and seminars

• Bóvári-Biri Judit
• Steinerbrunnerné Nagy Alexandra