Basics in Cell and Tissue Cultures

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The course material discusses the basic principles of cell culture techniques both for cell lines and primary tissues. Fundamental techniques for stem cell purification and stem cell culture will also be detailed. The course will provide both theoretical and practical background for the use of tissue cultures in complex pharmaceutical testing and biotechnological modification of cells and tissues.

Lectures

Practices

• 1. Practicing sterile work in a tissue culture laboratory, thawing up adhesive and non-adhesive cell lines - Bóvári-Biri Judit
• 2. Keeping cell lines in culture, passage of cell cultures, counting and pelletting cells, setting up cell cultures at set cell numbers - Bóvári-Biri Judit
• 3. Storage and freezing down cell lines. - Bóvári-Biri Judit
• 4. Practicing microbial screening I. - Steinerbrunnerné Nagy Alexandra
• 5. Practicing microbial screening II. - Steinerbrunnerné Nagy Alexandra
• 6. Testing cell lines for transdifferentiation, apoptosis, etc.. General fingerprinting of cell lines - Bóvári-Biri Judit
• 7. Designing immortalization of primary cells - Steinerbrunnerné Nagy Alexandra
• 8. Purification of primary cells using tissue characteristic markers - Steinerbrunnerné Nagy Alexandra
• 9. Culturing primary cells and testing survival and apoptotic death - Bóvári-Biri Judit
• 10. Purification of adult stem cells based on predicted markers - Bóvári-Biri Judit
• 11. Culturing stem cells - Bóvári-Biri Judit
• 12. Testing for differentiation markers and cellular proliferation - Bóvári-Biri Judit
• 13. Presentation and discussion: Individual experiments designed by students - Steinerbrunnerné Nagy Alexandra
• 14. Presentation and discussion: Individual experiments designed by students - Bóvári-Biri Judit

Seminars

• 1. History of cell cultures and introduction to a cell culture facility - Bóvári-Biri Judit
• 2. Cell and tissue types in culture. Origins of cell types, tissue banks - Bóvári-Biri Judit
• 3. Regulations on using cell cultures - Bóvári-Biri Judit
• 4. Cell culture protocols, risk assessment and microbial screening - Bóvári-Biri Judit
• 5. Preparatory techniques used in creating cell lines from complex tissues - Bóvári-Biri Judit
• 6. Differentiation and redifferentiation of cell lines. Testing spontaneous transformation. Regular fingerprinting - Bóvári-Biri Judit
• 7. Arteficially immortalized cell types - Bóvári-Biri Judit
• 8. Experimental designs using cell cultures - Steinerbrunnerné Nagy Alexandra
• 9. Main characteristics of stem cells and origin of stem cell types - Steinerbrunnerné Nagy Alexandra
• 10. Stem cell markers and stem cell cultures - Steinerbrunnerné Nagy Alexandra
• 11. Keeping stem cells in culture - Steinerbrunnerné Nagy Alexandra
• 12. Directed differentiation of stem cells - Steinerbrunnerné Nagy Alexandra
• 13. Complex stem cell cultures - Steinerbrunnerné Nagy Alexandra
• 14. Experimental designs using stem cells - Steinerbrunnerné Nagy Alexandra

Reading material

Obligatory literature

Literature developed by the Department

ppt

Notes

Basic and Complex cell and Tissue Culture techniques for Medical Biotechnology and Biotechnology MSc Students, PTE, 2014

Recommended literature

Bernice M. Martin: Fundamental Techniques in Cell Culture - a Laboratory Handbook (ECACC?), Tissue Culture Techniques: An Introduction, paperback, Aug 1, 1994

Kerry Atkinson: Clinical Bone Marrow and Blood Stem Cell Transplantation, 3rd edition, Cambridge University Press, 2004

Leda dos Reis Castilho: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Routledge, 2008

Clive Svendsen, Svendsen, Allison D. Ebert: Encyclopedia of Stem Cell Research, Sage eReference, SAGE Publications, 2008

Conditions for acceptance of the semester

Participation on seminars and practices

Mid-term exams

Written exam

Making up for missed classes

None

Exam topics/questions

Neptun MeetStreet, Teams

Examiners

Instructor / tutor of practices and seminars

• Bóvári-Biri Judit
• Garai Kitti
• Steinerbrunnerné Nagy Alexandra