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MISETA, Attila

MISETA, Attila

PhD, med.habil., DSc

full professor , director

Department of Laboratory Medicine

Telefon: 32120

Supervisor of the following TDK topics

Supervisor: MISETA, Attila

Co-supervisor: Dr. OSVÁTH, Péter

One of the oldest drug, lithium is still in use in the therapy of bipolar disorder. Since its molecular effects are not completely clarified, it is not known why some of the patients are “lithium responders” while some of them are “non-responders”. According to the literature and our previous results, lithium inhibits (among other enzymes) phosphoglucomutase (PGM). PGM catalyses the reversible conversion of glucose-1-P (Glc-1-P) to glucose-6-P (Glc-6-P). PGM is a key element in the main metabolic pathway of galactose, the lack of (the activity of) PGM results in low efficiency galactose metabolism.
The source of galactose in our food is almost exclusively milk and dairy-products. In our research we aim to explore whether there is a correlation between lithium’s therapy-efficiency and the amount of galactose consumption. Moreover we employ animal models to analyse lithium’s intracellular effect after various amount of galactose/glucose containing food intake.  

Supervisor: CZÉH, Boldizsár

Co-supervisor: Prof. Dr. MISETA, Attila

Neuropsychiatric disorders are common and often chronic illnesses imposing severe social and economic burden on our civilized societies. A further complicating issue is that often there are no valid biomarkers that could help us to make the proper diagnosis and to monitor the progression of the disease and the therapeutic response. However, it is important to search for novel, potential biomarkers and this is a field of intense clinical research. The applicant would join to a team of researches doing such clinical research and by that would also get familiar with the current diagnostic tools.

Supervisor: CZÉH, Boldizsár

Co-supervisor: Prof. Dr. MISETA, Attila

Adult hippocampal neurogenesis, a once unorthodox concept, has changed into one of the most rapidly growing fields in neuroscience. Numerous factors regulate adult neurogenesis including various neurotransmitters, hormones, stress, physical exercise learning and inflammation. Neurogenesis has been implicated in cognitive function and is stimulated by antidepressant drugs. A lasting reduction in neurogenesis following severe or chronic stress exposure, both in adult or early life, may represent impaired hippocampal plasticity and thus, can contribute to the cognitive symptoms of depression. Our aim is to understand the factors regulating this cellular process and to find novel approaches to stimulate this form of neural plasticity.

Supervisor: NAGY, Tamás

Co-supervisor: Prof. Dr. MISETA, Attila

Beneficial health effects of body exercise is common knowledge, however the nature of molecular mechanisms, intracellular processes that are influenced by frequent physical activity are far from completely understood. Exercise could be considered as a type of pre-conditioning; small-scale, controlled stress which induces adaptation mechanisms. The benefit of  preconditioning is that the body is prepared for a potential, more serious stress thus the tolerance level increases, moreover the chance of survival is significantly better.
Previously it was shown that hypoxic preconditioning elevates the level of protein O-Glycosylation which seems to be a beneficial effect for cell survival. Interestingly, O-Glycosylation is strongly linked to carbohydrate metabolism (and consequently, the energetic state of the cells) so it is possible that it is part of an adaptation mechanism that tries to control stress-induced deleterious processes.  In the present study, we aim to clarify whether frequent body exercise increases O-Glycosylation levels on proteins. 

Supervisor: NAGY, Tamás

Co-supervisor: Prof. Dr. MISETA, Attila

The 2 main molecular hallmarks of Alzheimer’s disease are the senile plaques (β-amyloid peptides) and neuro-fibrillary tangles (hyperphosphorylated tau proteins). Several studies demonstrated that chronic hypoglycemia that occurs more frequently in elderly people might be an significant cause of the disease. Due to lower glucose levels in the neurons, several post-translational modification requiring carbohydrates might change. E.g. O-Glycosylation, which competes for the same sites on protein with phosphorylation will be down-regulated, thus enabling tau proteins to be hyperphosphorylated. The ER stress and the so called “unfolded protein response” (UPR) is also associated with Alzheimer’s, which might be the consequence of aberrant N-glycans on both β-amyloid and tau.
The aim of the study is to better identify the various protein post-translational modifications that are altered due to hypoglycemia. Moreover, it is necessary to explore the impact of these changes on cellular functions such as viability, morphology, proliferation, etc. Lastly, the long term aim of the study is to find diagnostic markers that predict the development of Alzheimer’s at an early stage.  

Supervisor: CZÉH, Boldizsár

Co-supervisor: Prof. Dr. MISETA, Attila

Stress appears to be increasingly present in our modern, and demanding, industrialized society. Virtually every aspect of our body and brain can be influenced by stress and although its effects are partly mediated by powerful corticosteroid hormones that target the nervous system, relatively little is known about when, and how, the effects of stress shift from being beneficial and protective to becoming deleterious. Decades of stress research have provided valuable insights into whether stress can directly induce dysfunction and/or pathological alterations, which elements of stress exposure are responsible, and which structural substrates are involved. Our aim is to understand the neuropathological and molacular changes in response to stress in different CNS structures.

Supervisor: MISETA, Attila

Co-supervisor: Dr. NAGY, Tamás

The occurrence of bipolar disorder is about 1.2% for both sexes worldwide. Lithium is a frequently employed mood-stabilizer eventhough the exact molecular mechanism of its action is not known. One of its effects is replacing magnesium on proteins, including the enzyme called phosphoglucomutase (PGM). PGM, which is a key enzyme in galactose and glycogen metabolism, catalyses the reversible conversion of glucose-1-P (Glc-1-P) to glucose-6-P (Glc-6-P).
Interestingly, alterations in PGM activity also impact intracellular calcium regulation. We have shown earlier in a S. cerevisiae model that inhibiting PGM by lithium increased intracellular calcium storage, but decreased the calcium signals in excited cells. In the present study we will investigate the link between metabolic changes (PGM inhibition) and calcium regulation. According to our hypothesis and our preliminary results, the role of endoplasmic reticulum and protein post-translational modifications are key elements in this process.