Daten
Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2023-2024
Lehrbeauftragte/r
-
Dr. Katalin SIPOS
associate professor,
Department of Pharmaceutical Biology -
Semesterwochenstunden
Vorlesungen: 14
Praktika: 28
Seminare: 14
Insgesamt: 56
Fachangaben
- Kode des Kurses: OTV-SEBI-T
- 5 kredit
- Biotechnology BSc
- Specialised Core Module modul
- autumn
keine
Vizsgakurzus:ja
Zahl der Kursteilnehmer für den Kurs:
min. 1 – max. 24
Thematik
Students will learn the structure and the functions of the eukaryotic and prokaryotic cells. They will study the similarities and differences of them in order to understand how prokaryotes of yeast cells can be used for biotechnological purposes. We will study the basics of plant cells, as they serve as tools in biotechnology. Mammalian cells are studied in many aspects: structural components, protein transport, secretion, endocytosis, apoptosis, tumor generation, and using them as biotechnological tools. Students have to understand how cells are connecting and communication with each other, how cell signaling pathways are operation, as they are targets of many drugs.
Vorlesungen
- 1. Cells: common features and differences - Dr. Sipos Katalin
- 2. Prokaryotes and eukaryotes - Dr. Horváth Adrienn
- 3. Membrane structure - Jánosa Gergely
- 4. Structure of DNA and RNA - Dr. Pandur Edina
- 5. DNA replication - Pap Ramóna
- 6. DNA repair - Pap Ramóna
- 7. Transcription - Dr. Sipos Katalin
- 8. Translation - Dr. Pandur Edina
- 9. Posttranslational modifications. Protein breakdown. - Jánosa Gergely
- 10. Nucleus: structure and function. Mitochondria, ER, Golgi, peroxisomes. - Dr. Pandur Edina
- 11. Endocytosis, exocytosis, lysosomes. - Dr. Horváth Adrienn
- 12. Cytoskeleton - Dr. Pandur Edina
- 13. Cell signaling - Dr. Pandur Edina
- 14. Cell cycle and regulation - Dr. Sipos Katalin
Praktika
- 1. General instructions, laboratory rules, note books.
- 2. General instructions, laboratory rules, note books.
- 3. Bacteriology: general characterisation, selection markers, agar plates.
- 4. Bacteriology: general characterisation, selection markers, agar plates.
- 5. Evaluation of agar plate readings.
- 6. Types of protein concentration determination.
- 7. PCR
- 8. PCR
- 9. Nucleic acid separation on agarose gels.
- 10. Nucleic acid separation on agarose gels.
- 11. Microscopic differencies of basic cell types
- 12. Media used in cell culture experiments
- 13. Counting of cultured cells
- 14. Splitting of cultured cells
- 15. Determination of viability of cultured cells
- 16. Determination of viability of cultured cells
- 17. RNA isolation from cultured cells
- 18. RNA isolation from cultured cells
- 19. Real time PCR
- 20. mRNA expression analysis
- 21. Electron microscopy
- 22. Electron microscopy
- 23. DNA in forensic medicine
- 24. DNA in forensic medicine
- 25. ELISA methods in biology
- 26. ELISA methods in biology
- 27. Consultation
- 28. Consultation
Seminare
- 1. Chemical bonds, small molecules in the cell
- 2. Macromolecules of the cell. Structure and functions of proteins
- 3. Transport processes
- 4. Gene expression, levels of regulation
- 5. Chromosomes, mitosis
- 6. Viruses. DNA mutations
- 7. Maturation of primary transcripts
- 8. Regulation of translation, antibiotics
- 9. Protein interactions
- 10. Nuclear transport. Transport into mitochondria, ER, peroxisomes
- 11. Vesicular transport
- 12. Extracellular matrix, cell interactions
- 13. Tumor cells
- 14. Plant cells
Materialien zum Aneignen des Lehrstoffes
Obligatorische Literatur
Vom Institut veröffentlichter Lehrstoff
The materials of the lectures and seminars will appear on Neptune.
Skript
Empfohlene Literatur
G. M. Cooper, R. E. Hausman: The Cell: A Molecular Approach, Sinauer Associates, Inc., 7th Edition, 2016. ISBN: 9781605352909
Albert et al: Essential Cell Biology, ISBN-13: 978-0393680362
Voraussetzung zum Absolvieren des Semesters
Maximum of 25 % absence allowed
Semesteranforderungen
There is no written mid-term exam in the semester.
Möglichkeiten zur Nachholung der Fehlzeiten
According to personal agreement
Prüfungsfragen
There are no given exam questions. The topics of the exam will be the materials of lectures and seminars.
Prüfer
- Dr. Pandur Edina
- Dr. Sipos Katalin
- Jánosa Gergely
- Pap Ramóna
Praktika, Seminarleiter/innen
- Dr. Horváth Adrienn
- Dr. Pandur Edina
- Dr. Sipos Katalin
- Jánosa Gergely
- Pap Ramóna