Cell Biology

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

Students will learn the structure and the functions of the eukaryotic and prokaryotic cells. They will study the similarities and differences of them in order to understand how prokaryotes of yeast cells can be used for biotechnological purposes. We will study the basics of plant cells, as they serve as tools in biotechnology. Mammalian cells are studied in many aspects: structural components, protein transport, secretion, endocytosis, apoptosis, tumor generation, and using them as biotechnological tools. Students have to understand how cells are connecting and communication with each other, how cell signaling pathways are operation, as they are targets of many drugs.

Lectures

• 1. Cells: common features and differences - Dr. Sipos Katalin
• 2. Prokaryotes and eukaryotes - Dr. Horváth Adrienn
• 3. Membrane structure - Jánosa Gergely
• 4. Structure of DNA and RNA - Dr. Pandur Edina
• 5. DNA replication - Pap Ramóna
• 6. DNA repair - Pap Ramóna
• 7. Transcription - Dr. Sipos Katalin
• 8. Translation - Dr. Pandur Edina
• 9. Posttranslational modifications. Protein breakdown. - Jánosa Gergely
• 10. Nucleus: structure and function. Mitochondria, ER, Golgi, peroxisomes. - Dr. Pandur Edina
• 11. Endocytosis, exocytosis, lysosomes. - Dr. Horváth Adrienn
• 12. Cytoskeleton - Dr. Pandur Edina
• 13. Cell signaling - Dr. Pandur Edina
• 14. Cell cycle and regulation - Dr. Sipos Katalin

Practices

• 1. General instructions, laboratory rules, note books.
• 2. General instructions, laboratory rules, note books.
• 3. Bacteriology: general characterisation, selection markers, agar plates.
• 4. Bacteriology: general characterisation, selection markers, agar plates.
• 5. Evaluation of agar plate readings.
• 6. Types of protein concentration determination.
• 7. PCR
• 8. PCR
• 9. Nucleic acid separation on agarose gels.
• 10. Nucleic acid separation on agarose gels.
• 11. Microscopic differencies of basic cell types
• 12. Media used in cell culture experiments
• 13. Counting of cultured cells
• 14. Splitting of cultured cells
• 15. Determination of viability of cultured cells
• 16. Determination of viability of cultured cells
• 17. RNA isolation from cultured cells
• 18. RNA isolation from cultured cells
• 19. Real time PCR
• 20. mRNA expression analysis
• 21. Electron microscopy
• 22. Electron microscopy
• 23. DNA in forensic medicine
• 24. DNA in forensic medicine
• 25. ELISA methods in biology
• 26. ELISA methods in biology
• 27. Consultation
• 28. Consultation

Seminars

• 1. Chemical bonds, small molecules in the cell
• 2. Macromolecules of the cell. Structure and functions of proteins
• 3. Transport processes
• 4. Gene expression, levels of regulation
• 5. Chromosomes, mitosis
• 6. Viruses. DNA mutations
• 7. Maturation of primary transcripts
• 8. Regulation of translation, antibiotics
• 9. Protein interactions
• 10. Nuclear transport. Transport into mitochondria, ER, peroxisomes
• 11. Vesicular transport
• 12. Extracellular matrix, cell interactions
• 13. Tumor cells
• 14. Plant cells

Reading material

Obligatory literature

Literature developed by the Department

The materials of the lectures and seminars will appear on Neptune.

Notes

Recommended literature

G. M. Cooper, R. E. Hausman: The Cell: A Molecular Approach, Sinauer Associates, Inc., 7th Edition, 2016. ISBN: 9781605352909
Albert et al: Essential Cell Biology, ISBN-13: 978-0393680362

Conditions for acceptance of the semester

Maximum of 25 % absence allowed

Mid-term exams

There is no written mid-term exam in the semester.

Making up for missed classes

According to personal agreement

Exam topics/questions

There are no given exam questions. The topics of the exam will be the materials of lectures and seminars.

Examiners

• Dr. Pandur Edina
• Dr. Sipos Katalin
• Jánosa Gergely
• Pap Ramóna

Instructor / tutor of practices and seminars

• Dr. Horváth Adrienn
• Dr. Pandur Edina
• Dr. Sipos Katalin
• Jánosa Gergely
• Pap Ramóna