Biophysics - Lecture

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2026-2027

Thematik

The Biophysics curse aims to introduce students to the methods and applications, that are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR), imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI, CT), radioactive applications, calorimetry, and fast kinetics techniques. The lectures discuss in detail the physical bases and principles of each approach and the field of applications. The practices are dedicated to extending the students' knowledge and routine with the use of different techniques. The practices lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.

Vorlesungen

• 1.

  Introduction. Structure of the atom, structure of the atomic nucleus, quantum mechanical model of the atom. Molecular orbitals, LCAO (linear combination of atomic orbitals), energy levels (Jabłonski). Line-type spectra of atoms.

  - Ujfalusi Zoltán
• 2.

  Introduction. Structure of the atom, structure of the atomic nucleus, quantum mechanical model of the atom. Molecular orbitals, LCAO (linear combination of atomic orbitals), energy levels (Jabłonski). Line-type spectra of atoms.

  - Ujfalusi Zoltán
• 3.

  Electromagnetic waves, the electromagnetic spectrum. The wave and particle nature of light.

  - Ujfalusi Zoltán
• 4.

  Electromagnetic waves, the electromagnetic spectrum. The wave and particle nature of light.

  - Ujfalusi Zoltán
• 5.

  Introduction to protein engineering: Analytical separation techniques: chromatography, sedimentation, electrophoresis.

  - Pécsi Ildikó
• 6.

  Introduction to protein engineering: Analytical separation techniques: chromatography, sedimentation, electrophoresis.

  - Pécsi Ildikó
• 7.

  LASER

  - Talián Csaba Gábor
• 8.

  LASER

  - Talián Csaba Gábor
• 9.

  UV-VIS spectroscopy, absorption spectroscopy. Infrared and Raman spectroscopy.

  - Lukács András Szilárd
• 10.

  UV-VIS spectroscopy, absorption spectroscopy. Infrared and Raman spectroscopy.

  - Lukács András Szilárd
• 11.

  Fluorescence spectroscopy, Förster-type resonance energy transfer (FRET); Quenching, Rapid kinetic methods.

  - Lukács András Szilárd
• 12.

  Fluorescence spectroscopy, Förster-type resonance energy transfer (FRET); Quenching, Rapid kinetic methods.

  - Lukács András Szilárd
• 13.

  Basics of light microscopy. Fluorescence microscopy, FRAP.

  - Huberné Barkó Szilvia
• 14.

  Basics of light microscopy. Fluorescence microscopy, FRAP.

  - Huberné Barkó Szilvia
• 15.

  Modern microscopic methods (STORM, STED, SIM, confocal, 2-photon).

  - Szabó-Meleg Edina
• 16.

  Modern microscopic methods (STORM, STED, SIM, confocal, 2-photon).

  - Szabó-Meleg Edina
• 17.

  High resolution structural approaches: Generation of X-ray, X-ray crystallography, SAXS, cryo EM

  - Lukács András Szilárd
• 18.

  High resolution structural approaches: Generation of X-ray, X-ray crystallography, SAXS, cryo EM

  - Lukács András Szilárd
• 19.

  Thermodynamics, thermal analysis; Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC).

  - Pécsi Ildikó
• 20.

  Thermodynamics, thermal analysis; Calorimetric methods: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC).

  - Pécsi Ildikó
• 21.

  Flow cytometry. Fluorescence activated cell sorting (FACS).

  - Kirtania Prithwiraj
• 22.

  Flow cytometry. Fluorescence activated cell sorting (FACS).

  - Kirtania Prithwiraj
• 23.

  Spin. Nuclear magnetic resonance (NMR) spectroscopy. Magnetic resonance imaging (MRI).

  - Hild Gábor
• 24.

  Spin. Nuclear magnetic resonance (NMR) spectroscopy. Magnetic resonance imaging (MRI).

  - Hild Gábor
• 25.

  Medical imaging techniques in diagnostics 1. Ultrasound, Doppler ultrasound

  - Hild Gábor
• 26.

  Medical imaging techniques in diagnostics 1. Ultrasound, Doppler ultrasound

  - Hild Gábor
• 27.

  Medical imaging techniques in diagnostics 2. Gamma-camera, X-ray, computed tomography (CT), single-photon emission computed tomography (SPECT).

  - Hild Gábor
• 28.

  Medical imaging techniques in diagnostics 2. Gamma-camera, X-ray, computed tomography (CT), single-photon emission computed tomography (SPECT).

  - Hild Gábor

Praktika

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

All handouts and other related materials can be found in the corresponding Microsoft Teams group of the course.

Skript

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

There are no additional conditions.

Semesteranforderungen

Theory exams are scheduled in the exam period according to the rules of studies and examinations.

Möglichkeiten zur Nachholung der Fehlzeiten

The opportunity to make up for absence is not provided.

Prüfungsfragen

Topics of the exam questions:

Electromagnetic waves

Quantum numbers
NMR, MRI
Ultrasound

Structure and energy levels of atoms and molecules

Protein Structure. Introduction to protein engineering.

UV-VIS absorption spectroscopy

Fluorescence spectroscopy

Infrared and Raman spectroscopy

Flow cytometry

Light and fluorescence microscopy

Modern microscopic methods (STORM, STED, SIM, confocal, 2-photon)

Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)

Radioactivity, the interaction of radioactive radiations with matter

Biological effects of radioactive radiations, dosimetry

X-ray crystallography, SAXS, EM

Thermodynamics: laws and thermodynamic potentials

Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)

Analytical separation techniques: sedimentation, electrophoresis

Analytical separation techniques: chromatographic techniques

LASER

Prüfer

• Bukovics Péter
• Huberné Barkó Szilvia
• Lukács András Szilárd
• Szabó-Meleg Edina
• Takács-Kollár Veronika Tünde
• Ujfalusi Zoltán

Praktika, Seminarleiter/innen