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Ausbildung

Use of the EPR Spectroscopy on Biological Structures

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025

Lehrbeauftragte/r

Semesterwochenstunden

Vorlesungen: 6

Praktika: 6

Seminare: 0

Insgesamt: 12

Fachangaben

  • Kode des Kurses: OAF-EPR-T
  • 1 kredit
  • General Medicine
  • Optional modul
  • both
Voraussetzungen:

OAA-BI1-T finished

Zahl der Kursteilnehmer für den Kurs:

min. 5 – max. 6

Erreichbar als Campus-Kurs für . Campus-karok: ETK TTK

Thematik

The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.

The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.

The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Vorlesungen

  • 1. The basics of EPR Spectroscopy - Borbásné Farkas Kornélia
  • 2. The basic features of EPR Spectrometer - Borbásné Farkas Kornélia
  • 3. Spin labels and labeling methods - Dergez Tímea
  • 4. Free radicals - spin trapping - Dergez Tímea
  • 5. Membrane structures and their labeling - Dergez Tímea
  • 6. Other biological structures and their labeling - Dergez Tímea

Praktika

  • 1. Way of registration, registration and analyze of an EPR spectra - Dergez Tímea
  • 2. EPR spectra analysis of membrane dynamics 1. - Dergez Tímea
  • 3. EPR spectra analysis of membrane dynamics 2. - Dergez Tímea
  • 4. EPR spectra analysis of biological structures 1. (muscle fibres) - Dergez Tímea
  • 5. EPR spectra analysis of other biological structures 2. (with spin labelling technics) - Dergez Tímea
  • 6. Spin trapping method - Dergez Tímea

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

Notes in Microsoft Teams Group.

Skript

Empfohlene Literatur

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983

2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

Voraussetzung zum Absolvieren des Semesters

6 tasks to solve.

Semesteranforderungen

There are 6 homeworks (all with 5 short questions).

Möglichkeiten zur Nachholung der Fehlzeiten

Consultation

Prüfungsfragen

List of the exam can be find in Microsoft Teams Group.

Prüfer

Praktika, Seminarleiter/innen

  • Borbásné Farkas Kornélia
  • Dergez Tímea
Rólunk