Urinary Steroid Profiles

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2020-2021

Thematik

The participants are going to be familiarised theoretically and practically with:
- pre-treatment of urine sample
- qualitative and quantitative analysis of 24 androgen, progesterone and corticoid metabolites by gas chromatography using mass selective detector
- evaluation of urinary steroid profiles

Vorlesungen

• 1. Urinary steroid metabolites - Dr. Bufa Anita
• 2. Sample pre-treatment of urine - Dr. Bufa Anita
• 3. Gas chromatography-Mass spectrometry - Dr. Bufa Anita
• 4. Qualitative analysis of urinary steroid metabolites - Dr. Bufa Anita
• 5. Quantitative analysis of urinary steroid metabolites - Dr. Bufa Anita
• 6. Evaluation of urinary steroid profile - Dr. Bufa Anita

Praktika

• 1. Solid-phase extraction of urinary steroid metabolites
• 2. Enzymatic hydrolysis of urinary steroid metabolites
• 3. Derivatization of urinary steroid metabolites
• 4. Chromatographic cleaning
• 5. Determination of urinary steroid profiles by GC-MS technic
• 6. Qualitative and quantitative analysis of urinary steroid metabolites

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

It is going to be available on the website.

Skript

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

Maximum of 15 % absence allowed

Semesteranforderungen

After the course a written summary.

Möglichkeiten zur Nachholung der Fehlzeiten

Consultation with course director is required. The necessary information is going to be presented on the course.

Prüfungsfragen

The necessary information is going to be presented on the course.

Prüfer

Praktika, Seminarleiter/innen

• Dr. Bufa Anita