Possible Applications of Spectroscopic Methods Regarding the Investigation of Reversible Interactions

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2025-2026

Thematik

Optical spectroscopic methods are well suited for the study of reversible interactions (e.g., protein-ligand) of drugs, nutrients, toxins, and other xenobiotics. During the course, students will get acquainted with the theoretical and practical applications of widely used UV-visible absorption, fluorescence emission, and fluorescence anisotropy measurements, and will then learn the use of these methods in the study of various reversible interactions through concrete examples. The knowledge gained in this way promotes the understanding of measurement techniques and evaluation options and develops students' problem-solving skills and creativity in connection with spectroscopic examinations.

Vorlesungen

• 1. Description of the course requirements. Introduction: Optical spectroscopic methods. - Lemli Beáta
• 2. Theory of UV-visible spectrophotometry - Lemli Beáta
• 3. UV-visible spectrophotometry: examples of application - Lemli Beáta
• 4. Theory of fluorescence spectroscopy I. - Lemli Beáta
• 5. Theory of fluorescence spectroscopy II. - Lemli Beáta
• 6. Theory of fluorescence spectroscopy III. - Lemli Beáta
• 7. Theory of fluorescence spectroscopy IV. - Lemli Beáta
• 8. Characteristics of fluorescence emission: examples of application I. - Lemli Beáta
• 9. Characteristics of fluorescence emission: examples of application II. - Lemli Beáta
• 10. Fluorescence anisotropy: examples of application I. - Lemli Beáta
• 11. Fluorescence anisotropy: examples of application II. - Lemli Beáta
• 12. Summary and conclusions - Lemli Beáta
• 13. Written test - Lemli Beáta

Praktika

• 1. General instructions for work in the laboratory, safety precautions. - Lemli Beáta
• 2. UV-visible spectrophotometric measurement - Lemli Beáta
• 3. UV-visible spectrophotometric measurement - Lemli Beáta
• 4. UV-visible spectrophotometric measurement - Lemli Beáta
• 5. UV-visible spectrophotometric measurement - Lemli Beáta
• 6. Fluorescence spectroscopic measurement - fluorescence emission - Lemli Beáta
• 7. Fluorescence spectroscopic measurement - fluorescence emission - Lemli Beáta
• 8. Fluorescence spectroscopic measurement - fluorescence emission - Lemli Beáta
• 9. Fluorescence spectroscopic measurement - fluorescence emission - Lemli Beáta
• 10. Fluorescence spectroscopic measurement - anisotropy - Lemli Beáta
• 11. Fluorescence spectroscopic measurement - anisotropy - Lemli Beáta
• 12. Fluorescence spectroscopic measurement - anisotropy - Lemli Beáta
• 13. Fluorescence spectroscopic measurement - anisotropy - Lemli Beáta

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy, e.g. 3rd edition eBook ISBN 978-0-387-46312-4

Vom Institut veröffentlichter Lehrstoff

The slides of the lectures are electronically available.

Skript

Empfohlene Literatur

Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy, e.g. 3rd edition eBook ISBN 978-0-387-46312-4

Voraussetzung zum Absolvieren des Semesters

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Semesteranforderungen

One written exam on the 13th lecture, the acceptance level is 60%. The topics of the written test are similar to the topics of the lectures. An accepted report about the practacies (lab book).

Möglichkeiten zur Nachholung der Fehlzeiten

There is no possibility to make up for a missed lecture and/or practice.

Prüfungsfragen

The topics of the written test are similar to the topics of the lectures.

Prüfer

Praktika, Seminarleiter/innen

• Lemli Beáta