Extraction and Chromatographic Techniques in Medicinal Plant Analysis

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2020-2021

Thematik

With the increasing demand for herbal medicinal products, nutraceuticals, and natural products for health care all over the world, medicinal plant extract manufacturers and essential oil producers have started using the most appropriate extraction and chromatographic technologies in order to produce and standardize extracts and essential oils of defined quality. The aim of this course is to introduce the most important extraction methods and chromatographic techniques used in the research of medicinal plants. Students should be able to apply the acquired knowledge in their medical or pharmaceutical practice.
Task: To introduce state-of-the-art extraction techniques (maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression) and chromatographic analyses (column chromatography, thin layer chromatography, high pressure liquid chromatography) of medicinal plants and their role in the production of herbal medicines. Some applications, relating to different classes of substances, are presented to demonstrate the versatility of various chromatographic techniques.
Requirements: students have to know the most frequent extraction and analytical techniques used in medicinal and aromatic plant research.

Vorlesungen

• 1. Introduction of the course requirements. An Overview of Extraction Techniques for Medicinal and Aromatic Plants. - Dr. Bencsik Tímea
• 1. Chromatographic seminar: TLC. - Dr. Benkő András Antal
• 2. Introduction of state-of-the-art extraction techniques: maceration, infusion, digestion, decoction, percolation, Soxhlet, ultrasound extraction, supercritical fluid extraction, water and steam distillation, enfleurage, expression. - Dr. Bencsik Tímea
• 2. Chromatographic seminar: HPLC. - Dr. Bencsik Tímea
• 3. Introduction to Analytical Separations. - Dr. Benkő András Antal
• 4. Techniques for the Purposes of Botanical Research. - Dr. Benkő András Antal
• 5. Planar Chromatography: TLC. - Dr. Benkő András Antal
• 6. Role of Different Procedures and Test Systems in the Knowledge of Herbal Substances. - Dr. Benkő András Antal
• 7. Column Chromatography Systems: GC. - Dr. Benkő András Antal
• 8. Column Chromatography Systems: HPLC. - Dr. Benkő András Antal
• 9. Detectors Used in Different Separation Techniques and their Role in Qualitative and Quantitative Analysis. - Dr. Benkő András Antal
• 10. Classification of Detectors Based on their Sensitivity and Selectivity. - Dr. Benkő András Antal
• 11. Role of thin layer chromatography (TLC) in detecting microbiological processes: TLC-Bioautography. - Dr. Horváth Györgyi
• 12. Antimicrobial activity of essential oils using TLC-Bioautography. - Dr. Horváth Györgyi
• 13. Isolation and identification of carotenoids from medicinal plants. - Dr. Molnár Péter
• 14. Role of column liquid chromatography (CLC) in carotenoid research. - Dr. Molnár Péter
• 15. Examination of honeys, sugars, polyphenols. - Dr. Farkas Ágnes
• 16. Examination of honeys, sugars, polyphenols. - Dr. Farkas Ágnes
• 17. Identification of specific metabolites in herbal products. Adulterated dietary supplements. - Dr. Bencsik Tímea
• 18. Identification of specific metabolites in herbal products with TLC. - Dr. Bencsik Tímea
• 19. Isolation and Identification of flavonoids in medicinal plants. - Dr. Papp Nóra
• 20. Isolation and Identification of flavonoids in medicinal plants. - Dr. Papp Nóra
• 21. Quality of herbal products; our experiences in the Department of Pharmacognosy. - Dr. Bencsik Tímea
• 22. Quality of herbal products; our experiences in the Department of Pharmacognosy. - Dr. Bencsik Tímea
• 23. Student's lecture. - Dr. Bencsik Tímea
• 24. Student's lecture. - Dr. Bencsik Tímea
• 25. Written test. - Dr. Bencsik Tímea
• 26. Written test. - Dr. Bencsik Tímea

Praktika

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

PowerPoint presentations prepared by lecturers.

Skript

Empfohlene Literatur

1. Handa S.S., Khanuja S.P.S., Longo G., Rakesh D.D.: Extraction Technologies for Medicinal and Aromatic Plants, International Centre for Science and High Technology, Trieste, 2008
2. Reich E., Schibli A.: High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants, Thieme, UK, 2007
3. Colegate S.M.: Bioactive Natural Products Detection Isolation and Structural Determination, CRC Press, 2008
4. Niessen W.M.A.: Liquid Chromatography - Mass Spectrometry, CRC Press, 2006
5. Nyiredy, Sz.: Planar Chromatography, Springer, Budapest, 2001.
6. Harris, DC.: Quantitative Chemical Analysis, WH. Freeman and Co., 7th edition, New York, 2007,
7. Schiedt K., Liaaen-Jensen S.: Isolation and Analysis, in Carotenoids Vol. 1A: Isolation and Analysis, Eds.: Britton G., Liaaen-Jensen S., Pfander H., Birkhäuser Verlag, Basel, 1995.

Voraussetzung zum Absolvieren des Semesters

Maximum of 25 % absence allowed

Semesteranforderungen

One written exam during the semester, the acceptance level is 60%. One oral presentation by students.

Möglichkeiten zur Nachholung der Fehlzeiten

There is no possibility to make up for a missed lecture and practice.

Prüfungsfragen

The topics of the 1 written test are similar to the topics of the lectures.

Prüfer

Praktika, Seminarleiter/innen

• Dr. Bencsik Tímea
• Dr. Benkő András Antal