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Basic Immunology - Practice and Seminar

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 0 hours

practices: 14 hours

seminars: 14 hours

total of: 28 hours

Subject data

  • Code of subject: OBA-111-GS
  • 2 kredit
  • Biotechnology MSc
  • Basic modul
  • spring
Prerequisites:

OBA-111-E parallel

Course headcount limitations

min. 5 – max. 200

Topic

A Basic immunology előadáspkhoz kapcsolódó gyakorlatok

Lectures

Practices

  • 1. Introduction, structure of lymphoid organs.
  • 3. Immune defense against viruses, bacteria and fungi, diagnostic possibilities.
  • 5. Vaccines.
  • 7. Autoantibody diagnostics
  • 9. HLA typing.
  • 11. Consultation.
  • 13. Cells of the immune system, CD markers.
  • 15. Development of poly- and monoclonal antibodies and their applications. Immunization. Antibody production (fermentation), purification, labelling. Hybridoma technique.
  • 17. Immunocytochenistry, immunohistochemistry. Fluorescent microscopy.
  • 19. Flow cytometry. CD marker analysis. Isolation of white blood cells.
  • 21. Immunserology I.-II. Immunprecipitation, immundiffusion, immunELFO, agglutination, haemagglutination, Coombs-test.
  • 23. Seminar
  • 25. Immunserology III. ELISA, Dot-blot, Western-blot.
  • 27. Functional immunological tests: short term tissue cultures. Phagocytosis test. Cytotoxicity test. Lyphocyte activation, cytokine measurements.

Seminars

  • 2. Introduction, structure of lymphoid organs.
  • 4. Immune defense against viruses, bacteria and fungi, diagnostic possibilities.
  • 6. Vaccines.
  • 8. Autoantibody diagnostics
  • 10. HLA typing.
  • 12. Consultation.
  • 14. Cells of the immune system, CD markers.
  • 16. Development of poly- and monoclonal antibodies and their applications. Immunization. Antibody production (fermentation), purification, labelling. Hybridoma technique.
  • 18. Immunocytochenistry, immunohistochemistry. Fluorescent microscopy.
  • 20. Flow cytometry. CD marker analysis. Isolation of white blood cells.
  • 22. Immunserology I.-II. Immunprecipitation, immundiffusion, immunELFO, agglutination, haemagglutination, Coombs-test.
  • 24. Seminar
  • 26. Immunserology III. ELISA, Dot-blot, Western-blot.
  • 28. Functional immunological tests: short term tissue cultures. Phagocytosis test. Cytotoxicity test. Lyphocyte activation, cytokine measurements.

Reading material

Obligatory literature

minden 2010 után megjelent immunológia tankönyv

Literature developed by the Department

www.immbio.hu

Notes

nincs

Recommended literature

nincs

Conditions for acceptance of the semester

Részvétel

Mid-term exams

nincs

Making up for missed classes

nincs

Exam topics/questions

www.immbio.hu

Examiners

Instructor / tutor of practices and seminars

  • Böröcz Katalin
Rólunk