Molecular Targets - Signal Transduction - Practice

Data

Official data in SubjectManager for the following academic year: 2026-2027

Topic

To demonstrate basic laboratory techniques in signal transduction.

Lectures

Practices

• 1. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 2. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 3. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 4. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
• 5.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Isolation of murine lymphocytes and purification of cell populations. Activation of T cells with anti-CD3. Preparation of cell lysates for Western-blot.

• 6.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Isolation of murine lymphocytes and purification of cell populations. Activation of T cells with anti-CD3. Preparation of cell lysates for Western-blot.

• 7.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Isolation of murine lymphocytes and purification of cell populations. Activation of T cells with anti-CD3. Preparation of cell lysates for Western-blot.

• 8.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Isolation of murine lymphocytes and purification of cell populations. Activation of T cells with anti-CD3. Preparation of cell lysates for Western-blot.

• 9.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot.

• 10.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot.

• 11.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot.

• 12.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot.

• 13. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 14. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 15. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 16. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
• 17. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 18. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 19. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 20. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
• 21.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with phospho-flow.

• 22.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with phospho-flow.

• 23.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with phospho-flow.

• 24.

  Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with phospho-flow.

• 25.

  Overview of methods for studying transcription factors.

• 26.

  Overview of methods for studying transcription factors.

• 27.

  Overview of multiomics approach in signal transduction.

• 28.

  Overview of multiomics approach in signal transduction.

Seminars

Reading material

Obligatory literature

Gomperts: Signal Transduction

Literature developed by the Department

-

Notes

-

Recommended literature

Conditions for acceptance of the semester

none

Mid-term exams

Students have to prepare a summary about each practice and hand it in to the teacher.

Making up for missed classes

-

Exam topics/questions

-

Examiners

Instructor / tutor of practices and seminars

• KURZUSHOZ RENDELT OKTATÓ
• Olasz Katalin Eszter