Enzime Biotechnology

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The aim of the subject is to present the theoretical and practical background of enyzme biotechnology. The course discusses in detail the biological functions and practical use of enzymes in life sciences. Participants will become familiar with the possibilities of isolation, production and manipulation of enzymes together with their applications.

Lectures

• 1. Introduction to the subject (basic definitions, topics, requirements). - Boldizsár Ferenc
• 2. Introduction to the subject (division and characteristics of enzymes). - Boldizsár Ferenc
• 3. Nuclear enzymes 1. (basic characteristics) - Boldizsár Ferenc
• 4. Nuclear enzymes 2. (nucleic acid modifying enzymes) - Boldizsár Ferenc
• 5. Enzymes used in molecular biology 1. (DNA cleaving and ligating enzymes, DNA modifying enzymes) - Boldizsár Ferenc
• 6. Enzymes used in molecular biology 2. (Polymerases) - Boldizsár Ferenc
• 7. Serine proteases 1. - Boldizsár Ferenc
• 8. Serine proteases 2. - Boldizsár Ferenc
• 9. Enzymes of the complement system. - Boldizsár Ferenc
• 10. Diseases of the complement system. - Boldizsár Ferenc
• 11. Blood coagulation enzymes. - Boldizsár Ferenc
• 12. Disturbances of blood coagulation, classical and modern therapeutical approaches. - Boldizsár Ferenc
• 13. Enzymes in signaling 1. (Kinases) - Boldizsár Ferenc
• 14. Enzymes in signaling 2. (Phosphatases) - Boldizsár Ferenc
• 15. Targeting and modification of signaling enzymes. - Boldizsár Ferenc
• 16. Enzymes mediating apoptosis. - Boldizsár Ferenc
• 17. Alterations of the apoptotic enzymes and possible relations to diseases. - Boldizsár Ferenc
• 18. Mitochondrial enzymes 1. (basics) - Boldizsár Ferenc
• 19. Mitochondrial enzymes 2. (modifications, diseases) - Boldizsár Ferenc
• 20. Extracellular matrix enzymes 1. (basics, matrix metalloproteinases) - Boldizsár Ferenc
• 21. Extracellular matrix enzymes 2. (relation to malignant and autoimmune diseases, therapeutic targeting) - Boldizsár Ferenc
• 22. Lysosomal enzyme deficiencies, storage disorders and their biotechnological approaches 1. (glycogenoses) - Boldizsár Ferenc
• 23. Lysosomal enzyme deficiencies, storage disorders and their biotechnological approaches 2. (mucopolysacharidoses, sphingolipidoses) - Boldizsár Ferenc
• 24. Motor proteins (functions, diseases and biotechnological manipulation) - Boldizsár Ferenc

Practices

Seminars

Reading material

Obligatory literature

-

Literature developed by the Department

-

Notes

-

Recommended literature

1. Anthony J. Cichoke: The Complete Book of Enzyme Therapy: A Complete and Up-to-Date Reference to Effective Remedies, Penguin Putnam

2. Roger G. Melton and Richard J. Knox: Enzyme-Prodrug Strategies for Cancer Therapy, Kindle Edition

3. K. Buchholz, V. Kasche, and U.T. Bornscheuer: Biocatalysts and Enzyme Technology, Wiley, 2005

Conditions for acceptance of the semester

Maximum number of absences is 3.

Mid-term exams

One topic has to be worked out by each students individually and presented.

Making up for missed classes

No.

Exam topics/questions

-

Examiners

Instructor / tutor of practices and seminars