Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Ernszt Dávid
research associate professor,
1st Department of Internal Medicine -
Number of hours/semester
lectures: 12 hours
practices: 0 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OBF-BTP-T
- 1 kredit
- Biotechnology MSc
- Optional modul
- autumn
-
Exam course:Course headcount limitations
min. 5 – max. 25
Available as Campus course for . Campus-karok: ÁOK ÁJK BTK ETK KPVK GYTK KTK MK TTK
Topic
The aim of the course is to discuss molecular biology techmiques used in research. The topics mainly focuse on common DNA and genome editing techniques, fluorescent protein tagging and bioinformatic tools used in genome and image analysis.
Lectures
- 1.
Detection of specific nucleotide sequences
- Ernszt Dávid - 2.
Gene activity measurement (qPCR)
- Ernszt Dávid - 3. Online nucleotide databases - Ernszt Dávid
- 4. Primer design and qPCR analysis - Ernszt Dávid
- 5.
Molecular cloning
- Ernszt Dávid - 6.
Viral transduction and transfection techniques
- Ernszt Dávid - 7. Plasmid restriction mapping - Ernszt Dávid
- 8. In silico analysis - Ernszt Dávid
- 9.
Genome editing techniques
- Ernszt Dávid - 10.
CRISPR Cas9
- Ernszt Dávid - 11. Basics of data analysis - Ernszt Dávid
- 12. Basics of image analysis - Ernszt Dávid
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Topics of the lectures will be available in form of ppt files.
Notes
Recommended literature
Conditions for acceptance of the semester
-
Mid-term exams
Oral report based on the topics of lectures at the end of the course. Missed oral report can be catch up in that week.
Making up for missed classes
None
Exam topics/questions
Oral report based on the topics of lectures.