Basic Immunology

Data

Official data in SubjectManager for the following academic year: 2020-2021

Topic

Introduction to the current immunobiology. Construction of the human immune system: organs, tissues, cellular and molecular components. Organisation and regulation of immune functions: recognition, differentiation, effector functions and memory formation. Main aspects of the physiological and pathological immune functions. Basic immunopathological aspects of immune related diseases including autoimmune diseases, organ transplantation, allergy, immunity against tumors.

Lectures

• 1. Introduction. - Dr. Németh Péter János
• 2. Molecular components of the immune system. - Dr. Németh Péter János
• 3. Recognition molecules. Recognition by MHC. - Dr. Németh Péter János
• 4. Antigen presentation and MHC restriction. - Dr. Németh Péter János
• 5. Communication between the cellular components of the immune system. - Dr. Németh Péter János
• 6. Adhesion molecules, co-receptors, cytokines, cytokine receptors. - Dr. Németh Péter János
• 7. The complement system. - Dr. Berki Timea
• 8. Innate immunity: inflammation, leukocyte migration. - Dr. Engelmann Péter András
• 9. Organization and rearrangement of the antigen receptor genes. - Dr. Balogh Péter
• 10. Primary B cell development: developmental stages and checkpoints. - Dr. Balogh Péter
• 11. T-cell development in the thymus. Stages of maturation and the role of environmental factors. - Dr. Boldizsár Ferenc
• 12. Beginning of the immune response: early stages of T/B cell interaction, CD3 complex and signal transduction, BcR signal transduction. Co-stimulation. T-dependent - Dr. Boldizsár Ferenc
• 13. Components of the humoral immune response: extrafollicular reaction and germinal center. - Dr. Balogh Péter
• 14. Immunological memory. - Dr. Balogh Péter
• 15. Effector functions of immunoglobulins. Antigen-antibody reactions. IgE mediated immunereactions. - Dr. Berki Timea
• 16. Effector mechanisms of cell-mediated immune responses: 1. Cytotoxicity, 2. Th cell-mediated macrophage activation (Delayed type hypersensitivity=DTH) - Dr. Berki Timea
• 17. Allergies and hypersensitive reactions. - Dr. Boldizsár Ferenc
• 18. Allergies and hypersensitive reactions. - Dr. Boldizsár Ferenc
• 19. Systemic and local immunity (SALT, MALT). - Dr. Németh Péter János
• 20. Suppression of the immune response. - Dr. Németh Péter János
• 21. Immunological tolerance. - Dr. Németh Péter János
• 22. Autoimmunity and autoimmune diseases. - Dr. Németh Péter János
• 23. Primary immunodeficiencies. - Dr. Balogh Péter
• 24. Acquired immunodeficiencies, pathomechanism and progression of AIDS, diagnostic and therapeutic possibilities. - Dr. Najbauer József
• 25. Immunity against tumors. - Dr. Németh Péter János
• 26. Immunological aspects of organ transplantation. - Dr. Németh Péter János
• 27. Immuno-based therapies. - Dr. Németh Péter János
• 28. Protein therapies. Recombinant cytokines. Vaccine development. - Dr. Najbauer József

Practices

• 1. Introduction, structure of lymphoid organs.
• 2. Introduction, structure of lymphoid organs.
• 3. Immune defense against viruses, bacteria and fungi, diagnostic possibilities.
• 4. Immune defense against viruses, bacteria and fungi, diagnostic possibilities.
• 5. Vaccines.
• 6. Vaccines.
• 7. Autoantibody diagnostics
• 8. Autoantibody diagnostics
• 9. HLA typing.
• 10. HLA typing.
• 11. Consultation.
• 12. Consultation.
• 13. Cells of the immune system, CD markers.
• 14. Cells of the immune system, CD markers.
• 15. Development of poly- and monoclonal antibodies and their applications. Immunization. Antibody production (fermentation), purification, labelling. Hybridoma technique.
• 16. Development of poly- and monoclonal antibodies and their applications. Immunization. Antibody production (fermentation), purification, labelling. Hybridoma technique.
• 17. Immunocytochenistry, immunohistochemistry. Fluorescent microscopy.
• 18. Immunocytochenistry, immunohistochemistry. Fluorescent microscopy.
• 19. Flow cytometry. CD marker analysis. Isolation of white blood cells.
• 20. Flow cytometry. CD marker analysis. Isolation of white blood cells.
• 21. Immunserology I.-II. Immunprecipitation, immundiffusion, immunELFO, agglutination, haemagglutination, Coombs-test.
• 22. Immunserology I.-II. Immunprecipitation, immundiffusion, immunELFO, agglutination, haemagglutination, Coombs-test.
• 23. Seminar
• 24. Seminar
• 25. Immunserology III. ELISA, Dot-blot, Western-blot.
• 26. Immunserology III. ELISA, Dot-blot, Western-blot.
• 27. Functional immunological tests: short term tissue cultures. Phagocytosis test. Cytotoxicity test. Lyphocyte activation, cytokine measurements.
• 28. Functional immunological tests: short term tissue cultures. Phagocytosis test. Cytotoxicity test. Lyphocyte activation, cytokine measurements.

Seminars

Reading material

Obligatory literature

Literature developed by the Department

See on website www.immbio.hu

Notes

Recommended literature

Abul K. Abbas: Cellular and Molecular Immunology
Janis Kuby: Immunology
Jan Klein: Immunology

Conditions for acceptance of the semester

Prepared attendance on lectures and practices. No more absence as 3 in practices. Minimum 10 points in the tests during seminars/laboratory practices. The active attendance is controlled by online.

Mid-term exams

One chance to make up a small test during the practices.

Making up for missed classes

Practice performed with another group on the same week.

Exam topics/questions

See on web site www.immbio.hu. The written exam test might contain minimum requirement questions where the wrong answers could mean failure on the exam.

Examiners

• Dr. Balogh Péter
• Dr. Berki Timea
• Dr. Németh Péter János

Instructor / tutor of practices and seminars

• Dr. Balogh Péter
• Dr. Berki Timea
• Dr. Boldizsár Ferenc
• Dr. Engelmann Péter András
• Dr. Kellermayer Zoltán
• Dr. Najbauer József
• Dr. Németh Péter János