Immundiagnostical Methods

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

Demonstration of the general and specific characteristics of immunological methods used in routine clinical laboratory practice and research highlighting their indications and limitations. Practical demonstration of the most important immunoserological and cellular immunological techniques. Role of the immunological methods in research, diagnostics and therapy. Establishment of theoretical and practical laboratory diagnostic background for preclinical and clinical courses.

Lectures

• 1. Introduction into the laboratory diagnostic methods - Berki Timea
• 2. Immunchemistry, immunserology - Berki Timea
• 3. Cellular immunological methods, flow cytometry - Berki Timea
• 4. Detection of autoantibodies in systemic autoimmune diseases - Simon Diána
• 5. Autoantibodies in organ specific autoimmune diseases - Simon Diána
• 6. Immunphenotype analysis - Berki Timea
• 7. Disease associated HLA typing - Berki Timea
• 8. Multiplex detection methods - Simon Diána

Practices

• 1. Immunserology, ELISA - Berki Timea
• 2. Immunoblot, indirect immunfluorescence for autoantibody detection - Simon Diána
• 3. Phenotyping with flow cytometry - Berki Timea
• 4. Cytokine detection methods, functional test - Simon Diána

Seminars

Reading material

Obligatory literature

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Literature developed by the Department

www.immbio.hu

Notes

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Recommended literature

Abul K. Abbas: Cellular and Molecular Immunology

Conditions for acceptance of the semester

Presentation

Mid-term exams

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Making up for missed classes

Individual preparation.

Exam topics/questions

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Examiners

Instructor / tutor of practices and seminars

• Berki Timea
• Simon Diána