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Education

Molecular Development and Transdifferentation - Practice

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 0 hours

practices: 28 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBH-007-G
  • 2 kredit
  • Biotechnology MSc
  • Specialty in Medical Biotechnology modul
  • autumn
Prerequisites:

OBH-007-E parallel

Course headcount limitations

min. 5 – max. 25

Topic

Thje purpose of the subject is to provide laboratory training in basic embryological and stem-cell related analytical methods.

Lectures

Practices

  • 1. Alkaline phosphatase assay of JAR cells I. - Balogh Péter
  • 2. Alkaline phosphatase assay of JAR cells II. - Balogh Péter
  • 3. Invertebrate regeneration I - Engelmann Péter András
  • 4. Invertebrate regeneration II - Engelmann Péter András
  • 5. Histological analysis of invertebrate regeneration I - Engelmann Péter András
  • 6. Histological analysis of invertebrate regeneration II - Engelmann Péter András
  • 7. Isolation of eGFP bone marrow cells and hematopoietic reconstitution I - Balogh Péter
  • 8. Isolation of eGFP bone marrow cells and hematopoietic reconstitution II - Balogh Péter
  • 9. Visit to the IVF laboratory I - Gajdócsi Erzsébet Emilia
  • 10. Visit to the IVF laboratory II. - Gajdócsi Erzsébet Emilia
  • 11. Isolation of organs from mid-term mouse embryos I. - Balogh Péter
  • 12. Isolation of organs from mid-term mouse embryos II. - Balogh Péter
  • 13. In situ and ex vivo photoconversion of Kik photoprotein I. - Balogh Péter
  • 14. Isolation of organs from mid-term mouse embryos II. - Balogh Péter
  • 15. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac I. - Balogh Péter
  • 16. Phenotypic analysis of fetal organ-derived cultures: neural, liver, cardiac II. - Balogh Péter
  • 17. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice I. - Balogh Péter
  • 18. Analysis of hematopoietic stem cell engraftment in eGFP-transplanted mice II. - Balogh Péter
  • 19. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures I. - Gajdócsi Erzsébet Emilia
  • 20. Isolation pf preimplantation mouse embryos and initiation of embryonic stem cell cultures II. - Gajdócsi Erzsébet Emilia
  • 21. Passage of hatching embryos and expansion of eSC culture I. - Gajdócsi Erzsébet Emilia
  • 22. Passage of hatching embryos and expansion of eSC culture II. - Gajdócsi Erzsébet Emilia
  • 23. AP analysis of eSC cultures I. - Gajdócsi Erzsébet Emilia
  • 24. AP analysis of eSC cultures II. - Gajdócsi Erzsébet Emilia
  • 25. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells I. - Gajdócsi Erzsébet Emilia
  • 26. FACS sorting of tumor-inducing cells from Bc-DLFL.1 lymphoma cells II. - Gajdócsi Erzsébet Emilia
  • 27. Consultation - Engelmann Péter András
  • 28. Consultation - Gajdócsi Erzsébet Emilia

Seminars

Reading material

Obligatory literature

n/a

Literature developed by the Department

Handouts and protocols provided at each practice

Notes

n/a

Recommended literature

n/a

Conditions for acceptance of the semester

N/A

Mid-term exams

n/a

Making up for missed classes

none

Exam topics/questions

n/a

Examiners

Instructor / tutor of practices and seminars

  • Balogh Péter
  • Engelmann Péter András
Rólunk