Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Berki Timea
professor,
1st Department of Internal Medicine -
Number of hours/semester
lectures: 8 hours
practices: 4 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OBF-IMG-T
- 1 kredit
- Biotechnology MSc
- Optional modul
- autumn
-
Course headcount limitations
min. 5 – max. 20
Topic
Demonstration of the general and specific characteristics of immunological methods used in routine clinical laboratory practice and research highlighting their indications and limitations. Practical demonstration of the most important immunoserological and cellular immunological techniques. Role of the immunological methods in research, diagnostics and therapy. Establishment of theoretical and practical laboratory diagnostic background for preclinical and clinical courses.
Lectures
- 1. Introduction into the laboratory diagnostic methods - Berki Timea
- 2. Immunchemistry, immunserology - Berki Timea
- 3. Cellular immunological methods, flow cytometry - Berki Timea
- 4. Detection of autoantibodies in systemic autoimmune diseases - Simon Diána
- 5. Autoantibodies in organ specific autoimmune diseases - Simon Diána
- 6. Immunphenotype analysis - Berki Timea
- 7. Disease associated HLA typing - Berki Timea
- 8. Multiplex detection methods - Simon Diána
Practices
- 1. Immunserology, ELISA - Berki Timea
- 2. Immunoblot, indirect immunfluorescence for autoantibody detection - Simon Diána
- 3. Phenotyping with flow cytometry - Berki Timea
- 4. Cytokine detection methods, functional test - Simon Diána
Seminars
Reading material
Obligatory literature
-
Literature developed by the Department
www.immbio.hu
Notes
-
Recommended literature
Abul K. Abbas: Cellular and Molecular Immunology
Conditions for acceptance of the semester
Presentation
Mid-term exams
-
Making up for missed classes
Individual preparation.
Exam topics/questions
-
Examiners
Instructor / tutor of practices and seminars
- Berki Timea
- Simon Diána