Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
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Boldizsár Ferenc
associate professor,
1st Department of Internal Medicine -
Number of hours/semester
lectures: 0 hours
practices: 28 hours
seminars: 0 hours
total of: 28 hours
Subject data
- Code of subject: OBH-005-G
- 2 kredit
- Biotechnology MSc
- Specialty in Medical Biotechnology modul
- spring
Prerequisites:
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Course headcount limitations
min. 5 – max. 20
Topic
To demonstrate basic laboratory techniques in signal transduction.
Lectures
Practices
- 1. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
...
- 2. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
- 3. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
- 4. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
- 5. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
...
- 6. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 7. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 8. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 9. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 10. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 11. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 12. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
- 13. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
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- 14. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
- 15. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
- 16. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
- 17. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
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- 18. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
- 19. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
- 20. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
- 21. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
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- 22. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
- 23. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
- 24. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
- 25. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
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- 26. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
- 27. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
- 28. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
Seminars
Reading material
Obligatory literature
Gomperts: Signal Transduction
Literature developed by the Department
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Notes
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Recommended literature
Conditions for acceptance of the semester
none
Mid-term exams
Students have to prepare a summary about each practice and hand it in to the teacher.
Making up for missed classes
-
Exam topics/questions
-
Examiners
Instructor / tutor of practices and seminars
- Boldizsár Ferenc
- Olasz Katalin Eszter