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Molecular Targets – Signal Transduction - Practice

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 0 hours

practices: 28 hours

seminars: 0 hours

total of: 28 hours

Subject data

  • Code of subject: OBI-008-G
  • 2 kredit
  • Biotechnology MSc
  • Specialty in Pharmaceutical Biotechnology modul
  • spring
Prerequisites:

-

Course headcount limitations

min. 5 – max. 20

Topic

To demonstrate basic laboratory techniques in signal transduction.

Lectures

Practices

  • 1. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
  • ...
  • 2. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
  • 3. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
  • 4. Measurement of intracellular Ca level using flow cytometry. T cell activation with anti-CD3.
  • 5. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • ...
  • 6. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 7. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 8. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 9. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 10. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 11. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 12. Analysis of the phosphorylation status of the key signaling molecules in the TcR signaling pathway. Detection of tyrosine-phosphorylation in T cells after anti-CD3 activation with Western-blot or with phospho-flow technique using phospho-specific antibodies.
  • 13. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
  • ...
  • 14. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
  • 15. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
  • 16. Signaling pathways of apoptosis (intrinsic and extrinsic). AnnexinV/propidium-iodode staining.
  • 17. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
  • ...
  • 18. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
  • 19. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
  • 20. Signaling pathways of apoptosis (intrinsic and extrinsic). detection of activated Caspase-8, -9, -3.
  • 21. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
  • ...
  • 22. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
  • 23. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
  • 24. Signaling pathways of apoptosis (intrinsic and extrinsic). Analysis of the Bcl-2 protein family; changes of the mitochondrial membrane potential.
  • 25. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
  • ...
  • 26. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
  • 27. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.
  • 28. Analysis of the genomic and non-genomic glucocorticoid (GC) signaling pathways. Detection of the glucocorticoid-receptor using flow cytometry and microscopy.

Seminars

Reading material

Obligatory literature

Gomperts: Signal Transduction

Literature developed by the Department

-

Notes

-

Recommended literature

Conditions for acceptance of the semester

none

Mid-term exams

Students have to prepare a summary about each practice and hand it in to the teacher.

Making up for missed classes

-

Exam topics/questions

-

Examiners

Instructor / tutor of practices and seminars

  • Boldizsár Ferenc
  • Olasz Katalin Eszter
Rólunk