Data
Official data in SubjectManager for the following academic year: 2023-2024
Course director
-
Dr. Diána SIMON
associate professor,
Department of Immunology and Biotechnology -
Number of hours/semester
lectures: 14 hours
practices: 0 hours
seminars: 14 hours
total of: 28 hours
Subject data
- Code of subject: OBH-012-T
- 2 kredit
- Biotechnology MSc
- Specialty in Medical Biotechnology modul
- autumn
Prerequisites:
OBA-106-E completed
Exam course:yes
Course headcount limitations
min. 5 – max. 25
Topic
The modern laboratory diagnostic methods, including molecular and immunological techniques will be discussed.
Lectures
- 1. Basic terms of immunologic detection methods. Antigen-antibody reactions. Sensitivity, specificity of antigen-antibody reactions. Immunoassay types. - Dr. Berki Timea
- 2. Multiplex detection methods. Cytokine, chemokine detection methods. Biomarkers. - Dr. Berki Timea
- 3. Immunserological methods, autoantibody testing, detection of allergen specific IgE. - Dr. Simon Diána
- 4. The most frequently used analytical methods. Preanalytics, postanalytics. Laboratory automation. - Dr. Nagy Tamás
- 5. Plasma protein testing methods and evaluation of results in laboratory medicine. - Dr. Nagy Tamás
- 6. HLA and disease assotiation, methods for HLA-typing, HLA-B27 typing. Phage display. - Dr. Simon Diána
- 7. Molecular genetics in laboratory medicine - Dr. Gombos Katalin
- 8. Methods for locus-specific and genome-wide epigenetic studies - Dr. Rauch Tibor Attila
- 9. Genetic diagnostics of Mendelian disorders - Dr. Berenténé Dr. Bene Judit Ágnes
- 10. DNA and RNA sequencing technologies and applications - Urbán Péter
- 11. Molecular diagnostics of microbes. Nucleic Acid-based Techniques (NATs) in pathogen detection. - Dr. Pankovics Péter
- 12. Molecular diagnostics of microbes. Molecular typing methods in multi-drug resistant bacterial outbreak investigations. - Dr. Pál Ágnes (Dr. Sonnevend Ágnes Mária)
- 13. Cytogenetics in oncological diagnostics - Dr. Kajtár Béla
- 14. Molecular methods in precision oncology - Dr. Kajtár Béla
Practices
Seminars
- 1. Antigen-antibody reactions. Immunoassay types.
- 2. Multiplex detection methods
- 3. Autoantibody detection methods. Diagnostics of systemic autoimmune diseases. Laboratory diagnostics of allergy.
- 4. Immunchemistry laboratory equipments, detection methods.
- 5. Protein chemistry
- 6. HLA and diseases. HlA typing. Phage display libraries.
- 7. Genetics and molecular biology in laboratory medicine
- 8. Methods for epigenetic studies
- 9. Basic terms of genetic diagnostics. Genetic diagnostics of Mendelian disorders.
- 10. DNA and RNA sequencing technologies
- 11. Molecular diagnostics of microbes.
- 12. Molecular diagnostics of microbes
- 13. Cytogenetics in oncological diagnostics
- 14. Molecular testing in oncology
Reading material
Obligatory literature
Literature developed by the Department
www.medbiotech.com
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
-
Making up for missed classes
None
Exam topics/questions
Írásbeli tesztvizsga az oktatók által megadott kérdésekkel, szükség esetén online formában (moodle).
Examiners
- Dr. Simon Diána
Instructor / tutor of practices and seminars
- Dr. Berenténé Dr. Bene Judit Ágnes
- Dr. Berki Timea
- Dr. Gombos Katalin
- Dr. Kajtár Béla
- Dr. Nagy Tamás
- Dr. Pál Ágnes (Dr. Sonnevend Ágnes Mária)
- Dr. Pankovics Péter
- Dr. Rauch Tibor Attila
- Dr. Simon Diána
- Urbán Péter