Basics of Bloodtransfusion

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The aim of the subject is to provide the theoretical and practical basics necessary for transfusion therapy.

The topic of the subject: blood products, transfusion complications, ABO and RhD blood groups, blood grouping in theory and practice. The subject is based on physiological, pathophysiological, haematological, and emergency medical studies.

Number of classes: 3 hour lectures, 4 hour practices

Lecture

1. Presentation of blood, blood products and their indications

1.1. Place of transfusion medicine in medical sciences

1.2. Principle of transfusion

1.3. Theory of blood conservation

1.4. Production of blood products and their types

1.5. Administration of blood products, their indication and hazards

1.6. Future of transfusion medicine

2. Complications of transfusion

2.1. Transfusion guidelines - Who can perform transfusion?

2.2. Symptoms indicating transfusion side effects

2.3. Categorisation of transfusion complications

2.4. Symptoms, treatment, and prevention of transfusion complications

2.5. Infection transmission with blood products

2.6. Haemovigilance

3. Resource management in blood supply

A prerequisite of the hospitals safe supply with blood components is the conscious and efficient management of scarce resources. A most important such resource is the healthy, group-specific full-blood, the so called national blood treasure being beared by the national donor-pool. The management of the national donor-pool - with particular focus on the donor- and recipient age-trees changing and aging in different ways - is a most important task. The institutional (both blood-bank and hospital) management of the completed blood-components is impossible without an integrated information system that takes into consideration logistics, expiration and possible disposal of the preparations.

Practices

1. Practice - ABO blood group system - ABO blood grouping

(the test must be performed on two blood samples)

1.1. General theory: Elements of transfusiolon medicine: preparative and clinical transfusiology. Blood group serology - Immunhaematology

1.2. Theoretical studies relevant to practice

1.2.1. Concepts of antibody, antigen

1.2.2. Properties of red blood cell antigens

1.2.3. Properties of antibodies against red blood cell antigens

1.2.4. Landsteiner's rule

1.2.5. Agglutination reaction

1.2.6. Blood group serological test methods

1.2.7. Concept of clinical and laboratory blood grouping and their application

1.3. ABO blood grouping on slide

1.3.1. Signing of blood sample tubes, administration of slides

1.3.2. Preparation of cell suspensions (50% and 10%)

1.3.3. Test procedure

1.3.4. Sources of errors

1.3.5. Individual evaluation of test results

2. Practice - Rh blood group system, antibody tests - RhD blood grouping

(the test must be performed on two blood samples)

2.1. Theoretical overview

2.1.1. Antigens of the Rh system - Rh phenotype

2.1.2. Specificity of RhD antigen

2.1.3. Properties of antibodies against Rh antigens

2.2. RhD grouping on slide

2.2.1. Administration of blood sample tube and slide

2.2.2. Preparation of cell suspension (50%)

2.2.3. Test procedure

2.2.4. Sources of errors

2.2.5. Individual evaluation of test results

2.3. Bed side card usage

2.3.1. Purpose of bed side card

2.3.2. Principle of function

2.3.3. Test procedure

2.3.4. Sources of error

2.3.5. Individual evaluation of test results

2.4. Antibody tests - presentation

2.4.1. Elements and concept of compatibility

2.4.2. Purpose and principle of the test

2.4.3. Antibody screening and identification

2.4.4. Presentation of gel card method, evaluation of tests in small groups - discussion of results

Lectures

• 1. Basics of transfusion medicine: Presentation of blood, blood products and their indications - Csernus Zita
• 2. Basics of transfusion medicine: Complications of transfusion - Csernus Zita
• 3. Resource management in blood supply - Csernus Zita

Practices

• 1. Basics of transfusion medicine: ABO blood group system - ABO blood grouping
• 2. Basics of transfusion medicine: ABO blood group system - ABO blood grouping
• 3. Basics of transfusion medicine: Rh blood group system, antibody tests - RhD blood grouping
• 4. Basics of transfusion medicine: Rh blood group system, antibody tests - RhD blood grouping

Seminars

Reading material

Obligatory literature

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Literature developed by the Department

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Notes

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Recommended literature

http://aok.pte.hu/index.php?page=egyseg&egy_id=1910&nyelv=eng

National Blood Transfusion Service Pécs Regional Blood Transfusion Center Educational materials

Conditions for acceptance of the semester

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Mid-term exams

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Making up for missed classes

Additional practices at another group

Exam topics/questions

Included in the detailed thematics

Examiners

Instructor / tutor of practices and seminars

• Csernus Zita
• Faust Zsuzsanna