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Use of the EPR Spectroscopy on Biological Structures

Data

Official data in SubjectManager for the following academic year: 2025-2026

Course director

Number of hours/semester

lectures: 6 hours

practices: 6 hours

seminars: 0 hours

total of: 12 hours

Subject data

  • Code of subject: OSF-EPR-T
  • 1 kredit
  • Dentist
  • Optional modul
  • both
Prerequisites:

OSA-BZ1-T finished

Exam course:

Course headcount limitations

min. 5 – max. 6

Available as Campus course for . Campus-karok: TTK

Topic

The objective of this course is to evaluate the ensuing results of conventional techniques employed in electron paramagnetic resonance (EPR) spectroscopy on a range of samples. In addition to sharing numerous similarities with nuclear magnetic resonance (NMR) spectroscopy, EPR spectroscopy focuses on the properties of electron spin resonance. This technique provides data pertaining to the molecular structure and dynamics of diverse biological systems.

The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Lectures

  • 1.

    The basics of EPR Spectroscopy

    - Dergez Tímea
  • 2. The basic features of EPR Spectrometer - Borbásné Farkas Kornélia
  • 3. Spin labels and labeling methods - Dergez Tímea
  • 4. Free radicals - spin trapping - Dergez Tímea
  • 5. Membrane structures and their labeling - Dergez Tímea
  • 6. Other biological structures and their labeling - Dergez Tímea

Practices

  • 1. Way of registration, registration and analyze of an EPR spectra - Dergez Tímea
  • 2. EPR spectra analysis of membrane dynamics 1. - Dergez Tímea
  • 3. EPR spectra analysis of membrane dynamics 2. - Dergez Tímea
  • 4. EPR spectra analysis of biological structures 1. (muscle fibres) - Dergez Tímea
  • 5. EPR spectra analysis of other biological structures 2. (with spin labelling technics) - Dergez Tímea
  • 6. Spin trapping method - Dergez Tímea

Seminars

Reading material

Obligatory literature

Literature developed by the Department

The teaching method provides notes and slides for each lesson, which are then sended to students via Moodle or Teams.

Notes

Recommended literature

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983

2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

Conditions for acceptance of the semester

In order to successfully complete the course, it is necessary to submit six pieces of homework that have been completed to a standard which allows for evaluation.

Mid-term exams

There are 6 homeworks (all with 5 short questions).

Making up for missed classes

Consultation

Exam topics/questions

It is important to note that no exam questions are included in this course. Instead, there are other conditions that must be met in order to successfully complete the course.

Examiners

Instructor / tutor of practices and seminars

  • Borbásné Farkas Kornélia
  • Dergez Tímea
Rólunk