Use of the EPR Spectroscopy on Biological Structures

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2025-2026

Tematika

The objective of this course is to evaluate the ensuing results of conventional techniques employed in electron paramagnetic resonance (EPR) spectroscopy on a range of samples. In addition to sharing numerous similarities with nuclear magnetic resonance (NMR) spectroscopy, EPR spectroscopy focuses on the properties of electron spin resonance. This technique provides data pertaining to the molecular structure and dynamics of diverse biological systems.

The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Előadások

• 1.

  The basics of EPR Spectroscopy

  - Dergez Tímea
• 2. The basic features of EPR Spectrometer - Borbásné Farkas Kornélia
• 3. Spin labels and labeling methods - Dergez Tímea
• 4. Free radicals - spin trapping - Dergez Tímea
• 5. Membrane structures and their labeling - Dergez Tímea
• 6. Other biological structures and their labeling - Dergez Tímea

Gyakorlatok

• 1. Way of registration, registration and analyze of an EPR spectra - Dergez Tímea
• 2. EPR spectra analysis of membrane dynamics 1. - Dergez Tímea
• 3. EPR spectra analysis of membrane dynamics 2. - Dergez Tímea
• 4. EPR spectra analysis of biological structures 1. (muscle fibres) - Dergez Tímea
• 5. EPR spectra analysis of other biological structures 2. (with spin labelling technics) - Dergez Tímea
• 6. Spin trapping method - Dergez Tímea

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

The teaching method provides notes and slides for each lesson, which are then sended to students via Moodle or Teams.

Jegyzet

Ajánlott irodalom

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983

2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

A félév elfogadásának feltételei

In order to successfully complete the course, it is necessary to submit six pieces of homework that have been completed to a standard which allows for evaluation.

Félévközi ellenőrzések

There are 6 homeworks (all with 5 short questions).

Távolmaradás pótlásának lehetőségei

Consultation

Vizsgakérdések

It is important to note that no exam questions are included in this course. Instead, there are other conditions that must be met in order to successfully complete the course.

Vizsgáztatók

Gyakorlatok, szemináriumok oktatói

• Borbásné Farkas Kornélia
• Dergez Tímea