Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Sipos Katalin
associate professor,
-
Number of hours/semester
lectures: 14 hours
practices: 0 hours
seminars: 0 hours
total of: 14 hours
Subject data
- Code of subject: OBI-011-T
- 1 kredit
- Biotechnology MSc
- Specialty in Pharmaceutical Biotechnology modul
- autumn
-
Course headcount limitations
min. 1 – max. 100
Topic
It is necessary to produce proteins for therapeutic and diagnostic purposes in good quality, reproducibly, and in large scale. For these aims the proper host cells, the recombinant technology, the purification methods, the probable modifications and the quality control of the protein products have to be chosen. The students will study the methods of large scale production of recombinant proteins, the possibilities of protein engineering and purifications. These studies can be the bases of their future experimental works.
Lectures
- 1.
Relationship of protein structure and function
- Sipos Katalin - 2. Major differences between prokaryotic and eukaryotic protein syntheses - Pandur Edina
- 3.
Recombinant protein production in general.
- Jánosa Gergely - 4.
Heterologous protein production in bacteria
- Sipos Katalin - 5. Heterologous protein production in cell culture. - Pandur Edina
- 6.
Proteins from plants and animal tissues.
- Jánosa Gergely - 7. Large scale protein production - Pap Ramóna
- 8.
Protein purification methods for expressed proteins.
- Sipos Katalin - 9.
Characterization of purified proteins
- Sipos Katalin - 10.
Posttranslational modifications.
Folding of recombinant proteins.
- Jánosa Gergely - 11.
Examples of human therapeutic proteins and enzymes
- Sipos Katalin - 12. Vaccine technology - Pap Ramóna
- 13.
Quality control
- Jánosa Gergely - 14.
Industrial enzymes: introduction.
Special industrial enzymes.
- Pandur Edina
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
The materials of the lectures will appear on Neptune.
Notes
Recommended literature
Walsh, G.: Proteins: Biochemistry and Biotechnology (2014)
Conditions for acceptance of the semester
There are no other terms of acceptance.
Mid-term exams
The course will end with a written exam.
Making up for missed classes
According to personal agreement
Exam topics/questions
There are no exam questions given. The topics of the exam will be the materials of lectures.
Examiners
- Pandur Edina
- Pap Ramóna
- Sipos Katalin