Protein Biotechnology

Data

Official data in SubjectManager for the following academic year: 2024-2025

Course director

Number of hours/semester

lectures: 14 hours

practices: 0 hours

seminars: 0 hours

total of: 14 hours

Subject data

  • Code of subject: OBI-011-T
  • 1 kredit
  • Biotechnology MSc
  • Specialty in Pharmaceutical Biotechnology modul
  • autumn
Prerequisites:

-

Course headcount limitations

min. 1 – max. 100

Topic

It is necessary to produce proteins for therapeutic and diagnostic purposes in good quality, reproducibly, and in large scale. For these aims the proper host cells, the recombinant technology, the purification methods, the probable modifications and the quality control of the protein products have to be chosen. The students will study the methods of large scale production of recombinant proteins, the possibilities of protein engineering and purifications. These studies can be the bases of their future experimental works.

Lectures

  • 1.

    Relationship of protein structure and function

    - Sipos Katalin
  • 2. Major differences between prokaryotic and eukaryotic protein syntheses - Pandur Edina
  • 3.

    Recombinant protein production in general.

    - Jánosa Gergely
  • 4.

    Heterologous protein production in bacteria

    - Sipos Katalin
  • 5. Heterologous protein production in cell culture. - Pandur Edina
  • 6.

    Proteins from plants and animal tissues.

    - Jánosa Gergely
  • 7. Large scale protein production - Pap Ramóna
  • 8.

    Protein purification methods for expressed proteins.

    - Sipos Katalin
  • 9.

    Characterization of purified proteins

    - Sipos Katalin
  • 10.

    Posttranslational modifications.

    Folding of recombinant proteins.

    - Jánosa Gergely
  • 11.

    Examples of human therapeutic proteins and enzymes

    - Sipos Katalin
  • 12. Vaccine technology - Pap Ramóna
  • 13.

    Quality control

    - Jánosa Gergely
  • 14.

    Industrial enzymes: introduction.

    Special industrial enzymes.

    - Pandur Edina

Practices

Seminars

Reading material

Obligatory literature

Literature developed by the Department

The materials of the lectures will appear on Neptune.

Notes

Recommended literature

Walsh, G.: Proteins: Biochemistry and Biotechnology (2014)

Conditions for acceptance of the semester

There are no other terms of acceptance.

Mid-term exams

The course will end with a written exam.

Making up for missed classes

According to personal agreement

Exam topics/questions

There are no exam questions given. The topics of the exam will be the materials of lectures.

Examiners

  • Pandur Edina
  • Pap Ramóna
  • Sipos Katalin

Instructor / tutor of practices and seminars