Protein Biotechnology

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2024-2025

Tematika

It is necessary to produce proteins for therapeutic and diagnostic purposes in good quality, reproducibly, and in large scale. For these aims the proper host cells, the recombinant technology, the purification methods, the probable modifications and the quality control of the protein products have to be chosen. The students will study the methods of large scale production of recombinant proteins, the possibilities of protein engineering and purifications. These studies can be the bases of their future experimental works.

Előadások

• 1.

  Relationship of protein structure and function

  - Sipos Katalin
• 2. Major differences between prokaryotic and eukaryotic protein syntheses - Pandur Edina
• 3.

  Recombinant protein production in general.

  - Jánosa Gergely
• 4.

  Heterologous protein production in bacteria

  - Sipos Katalin
• 5. Heterologous protein production in cell culture. - Pandur Edina
• 6.

  Proteins from plants and animal tissues.

  - Jánosa Gergely
• 7. Large scale protein production - Pap Ramóna
• 8.

  Protein purification methods for expressed proteins.

  - Sipos Katalin
• 9.

  Characterization of purified proteins

  - Sipos Katalin
• 10.

  Posttranslational modifications.

  Folding of recombinant proteins.

  - Jánosa Gergely
• 11.

  Examples of human therapeutic proteins and enzymes

  - Sipos Katalin
• 12. Vaccine technology - Pap Ramóna
• 13.

  Quality control

  - Jánosa Gergely
• 14.

  Industrial enzymes: introduction.

  Special industrial enzymes.

  - Pandur Edina

Gyakorlatok

Szemináriumok

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

The materials of the lectures will appear on Neptune.

Jegyzet

Ajánlott irodalom

Walsh, G.: Proteins: Biochemistry and Biotechnology (2014)

A félév elfogadásának feltételei

There are no other terms of acceptance.

Félévközi ellenőrzések

The course will end with a written exam.

Távolmaradás pótlásának lehetőségei

According to personal agreement

Vizsgakérdések

There are no exam questions given. The topics of the exam will be the materials of lectures.

Vizsgáztatók

• Pandur Edina
• Pap Ramóna
• Sipos Katalin

Gyakorlatok, szemináriumok oktatói