Microbiology 2 - Practice

Data

Official data in SubjectManager for the following academic year: 2026-2027

Topic

The primary objective of this practical course is to enable students to master the fundamental laboratory techniques required for the safe handling of microorganisms. Proficiency in aseptic techniques provides essential skills that are indispensable for pharmaceutical activities, directly supporting the goal of ensuring access to safe medicines.

The training places significant emphasis on the diagnostic background of antimicrobial therapy, which is a cornerstone in the global fight against antimicrobial resistance. Knowledge of antimicrobial susceptibility testing, measurement of antibiotic concentrations in body fluids, and the evaluation of antimicrobial combinations empowers pharmacists to act as consultants within multidisciplinary teams in the effective management of infectious diseases.

Understanding the theory and practice of sterility and pyrogen testing, demonstrated during the course, serves as the foundation for safe drug supply and a comprehensive pharmaceutical quality assurance mindset. Furthermore, students become familiar with key laboratory methods and gain an overview of their roles in microbiological diagnostics. Equipped with this knowledge, future pharmacists will be capable of critically interpreting microbiological laboratory reports and supporting targeted, rational antimicrobial therapy in patient care.

Lectures

Practices

• 3.

  Microscopic examinations, staining techniques

  - Tigyi Zoltán
• 4.

  Microscopic examinations, staining techniques

  - Tigyi Zoltán
• 5.

  Cultivation of bacteria, culture media and techniques.

  - Melegh Szilvia Zsóka
• 6.

  Cultivation of bacteria, culture media and techniques.

  - Melegh Szilvia Zsóka
• 7.

  Identification of bacteria

  - Melegh Szilvia Zsóka
• 8.

  Identification of bacteria

  - Melegh Szilvia Zsóka
• 17.

  Serological methods in microbiology

  - Meggyes Mátyás
• 18.

  Serological methods in microbiology

  - Meggyes Mátyás
• 19.

  Molecular methods in microbiology

  - Pankovics Péter
• 20.

  Molecular methods in microbiology

  - Pankovics Péter
• 16.

  Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora

  - Kocsis Béla
• 15.

  Sterility and pyrogen testing methods. Demonstration of the effect of disinfectants on skin flora

  - Kocsis Béla
• 9.

  Antimicrobial susceptibility testing

  - Melegh Szilvia Zsóka
• 10.

  Antimicrobial susceptibility testing

  - Melegh Szilvia Zsóka
• 27.

  Assessment

  - Melegh Szilvia Zsóka
• 28.

  Assessment

  - Melegh Szilvia Zsóka
• 1.

  General safety regulations, aseptic technique

  - Melegh Szilvia Zsóka
• 2.

  General safety regulations, aseptic technique

  - Melegh Szilvia Zsóka
• 11.

  Enumeration of microorganism

  - Melegh Szilvia Zsóka
• 12.

  Enumeration of microorganism

  - Melegh Szilvia Zsóka
• 21.

  Microbiological diagnostics of bacterial infections

  - Melegh Szilvia Zsóka
• 22.

  Microbiological diagnostics of bacterial infections

  - Melegh Szilvia Zsóka
• 13.

  Measurement of antibiotic concentration in body fluids, evaluation of antimicrobial combinations

  - Melegh Szilvia Zsóka
• 14.

  Measurement of antibiotic concentration in body fluids, evaluation of antimicrobial combinations

  - Melegh Szilvia Zsóka
• 26.

  Microbiological diagnostics of fungal and parasitic infections

  - Hargitai Renáta Nóra
• 25.

  Microbiological diagnostics of fungal and parasitic infections

  - Hargitai Renáta Nóra
• 23.

  Microbiological diagnostics of viral infections

  - Boros Ákos
• 24.

  Microbiological diagnostics of viral infections

  - Boros Ákos

Seminars

Reading material

Obligatory literature

Denyer SP ,Hodges NA, Gorman SP, Gilmore BF: Hugo and Russells Pharmaceutical Microbiology, 8th Edition, Blackwell Science Ltd

2018. ISBN: 978-1-119-43449-8

Tim Sandle: Pharmaceutical Microbiology : Essentials for Quality Assurance and Quality Control, ELSEVIER SCIENCE & TECHNOLOGY ,

Woodhead Publishing Ltd, 2015 ISBN10 0081000227

Literature developed by the Department

Practical materials on Teams

Notes

-

Recommended literature

Dr Patrick R. Murray, Dr Ken S. Rosenthal and Dr Michael A. Pfaller (eds.): Medical Microbiology, 9th edition, Elsevier Saunders 2020 ISBN13 (EAN): 9780323673228

Conditions for acceptance of the semester

Regular attendance of classes (a maximum of 3 missed weeks can be accepted) and earning a passing grade (grade 2-5) on the end-of-term assessment is required for accepting the semester.

Mid-term exams

The end-of-term assessment will be an oral exam held at the regular time of the class on the last week of the semester. In case of an unsuccessful end-of-term assessment, a retake opportunity is granted following personal discussion with the course director.

Making up for missed classes

Consultation with the supervisor.

Exam topics/questions

1. Aseptic Technique

2. Gram Staining

3. Bacterial culture: media methods

4. Bacterial identification methods

5. Disk diffusion testing

6. Determination of Minimum Inhibitory Concentration (MIC)

7. Microbial enumeration techniques

8. Measurement of antibiotic concentration in body fluids

9. Evaluation of antimicrobial combinations

10. Sterility testing

11. Pyrogen testing
12. Serological methods in microbiology

13. Molecular methods in microbiology
14. Microbiological diagnostics of bacterial infections

15. Microbiological diagnostics of viral infections

16. Microbiological diagnostics of fungal and parasitic infections

Examiners

Instructor / tutor of practices and seminars

• Boros Ákos
• Hargitai Renáta Nóra
• Kocsis Béla
• Meggyes Mátyás
• Melegh Szilvia Zsóka
• Pankovics Péter
• Polgár Beáta
• Tigyi Zoltán