Biotechnology on the Border of Physics and Chemistry

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

Students learn the theoretical background and the related application of several spectroscopic and microscopic methods including e.g. ESR, Raman, AFM, STM, SERS, TERS, SEM, etc. Several examples from the related recent research are presented in details. Students will have ability for successful application of the related measuring and data-evaluating methods too. The curse gives considerable support to earn the characterization and designer attitudes of students which then can be applied during their career in biotechnology.

Lectures

• 1. Types of Spectroscopy. Definition, examples, applications. Samples, models, evaluation. - Kunsági-Máté Sándor
• 2. Electron spin resonance (ESR) spectroscopy in biotechnology. Samples and evaluation tools. - Kunsági-Máté Sándor
• 3. Application example: Comparative EPR Study on the Scavenging Effect of Methotrexate with the Isomers of Its Photo-switchable Derivative - Kunsági-Máté Sándor
• 4. Nuclear magnetic resonance (NMR) spectroscopy. Solid-state NMR spectroscopy as a tool to determine the molecular structure of solids. - Kunsági-Máté Sándor
• 5. Jablonski scheme of molecules: Absorption, fluorescence, polarization, anisotropy, lifetime, anisotropy decay, solvent – relaxation, optical activity. - Kunsági-Máté Sándor
• 6. Application example of fluorescence spectroscopy: Determining the Weak Interactions of the Isomers of Phototrexate and Two Cavitand Derivatives; - Kunsági-Máté Sándor
• 7. Application example of fluorescence polarization : Quantitation of species differences in albumin–ligand interactions for bovine, human and rat serum albumins. - Kunsági-Máté Sándor
• 8. Raman spectroscopy. Application examples: a) Polymerization of dental composites. b) Adsorption of carbon nanotubes onto the substrate surfaces. - Kunsági-Máté Sándor
• 9. Molecular symmetries, group theory and consequences in spectroscopy. - Kunsági-Máté Sándor
• 10. Near-field spectroscopies: Surface – enhanced Raman spectroscopy. - Kunsági-Máté Sándor
• 11. Near-field microscopies: Scanning Electron Microscopy, Scanning Tunneling Microscopy, Atomic Force Microscopy, Electrostatic Force Microscopy, Scanning Capacitance Microscopy. - Kunsági-Máté Sándor
• 12. Microelectrodes. Scanning Electrochemical Microscopy. - Kiss László
• 13. Tip-enhanced Raman spectroscopy: a combination of the AFM and Raman equipment. - Kunsági-Máté Sándor
• 14. Application example: characterization of carbon nanostructures on different substrates. - Kunsági-Máté Sándor

Practices

• 1. General information, safety regulations, laboratory tools and equipments. - Kiss László
• 2. General information, safety regulations, laboratory tools and equipments. - Kiss László
• 3. Immobilisation of an organic layer by electrochemical copolymerization and its use in detection of aromatics - Kiss László
• 4. Immobilisation of an organic layer by electrochemical copolymerization and its use in detection of aromatics - Kiss László
• 5. Determination of critical concentration for micelle formation by conductometry - Kiss László
• 6. Determination of critical concentration for micelle formation by conductometry - Kiss László
• 7. Determination of degree of dissociation of pyruvic acid with freezing point decrease and its use for concentration measurements - Kiss László
• 8. Determination of degree of dissociation of pyruvic acid with freezing point decrease and its use for concentration measurements - Kiss László
• 9. Determination of the selectivity coefficient of an ion selective electrode - Kiss László
• 10. Determination of the selectivity coefficient of an ion selective electrode - Kiss László
• 11. Spectrophotometric investigation of Metanil Yellow in absence and presence of nitrite ions - Kiss László
• 12. Spectrophotometric investigation of Metanil Yellow in absence and presence of nitrite ions - Kiss László
• 13. Investigation of the water-acetic acid-ethyl acetate ternary system - Kiss László
• 14. Investigation of the water-acetic acid-ethyl acetate ternary system - Kiss László

Seminars

Reading material

Obligatory literature

1. P. W. Atkins, J. de Paula: Physical Chemistry, 11th edition, 2018, Oxford University Press, ISBN-13: 978-0198769866, ISBN-10: 0198769865

2. P. W. Atkins, J. de Paula: Physical chemistry for life sciences, 2006, Oxford University Press

Literature developed by the Department

Slides and notes of all lectures are available electronically.

Notes

Recommended literature

K.W. Kolasinski: Physical Chemistry: How Chemistry Works 1st Ed, 2016, Wiley, ISBN-13: 978-1118751121, ISBN-10: 1118751124

Conditions for acceptance of the semester

Written tests in the practice classes should pass the criteria.

Mid-term exams

Written test covering the topics of the lectures and the seminars. The result of the first part of the written exam (Minimum Written Test) should be at least 80%. In the case of the third exam the written exam is evaluated regardless the result of the Minimum Written Test. The list of the possible questions of the Minimum Written Test is announced on the Neptun system. The result of the written exam must be above 60%. The final grade is based on results of the midterm tests and the written exam. Maximum contribution of the results of the midterm tests to the total score of the written exam is 25%. Participation on the first exam is compulsory. The result of the written test must be above 60%. The final grade is based on results of the midterm tests and the written test. Maximum contribution of the results of the midterm tests to to the total score of the written test can be 25%. Participation on the first exam is compulsory.

Making up for missed classes

There is no opportunity to make up missed classes (lectures).

Exam topics/questions

Electron spin resonance (ESR) spectroscopy, Samples and evaluation tools.

Scavenging Effect of Methotrexate with the Isomers of Its Photo-switchable Derivative

Nuclear magnetic resonance (NMR) spectroscopy. Solid-state NMR spectroscopy as a tool to determine the molecular structure of solids.

Optical spectroscopies: Absorption, fluorescence, polarization, anisotropy, lifetime, anisotropy decay, solvent – relaxation, optical activity.

Raman spectroscopy and applications

Molecular symmetries, group theory and consequences in spectroscopy.

Near-field spectroscopies: Surface – enhanced Raman spectroscopy.

Near-field microscopies: Scanning Electron Microscopy, Scanning Tunneling Microscopy, Atomic Force Microscopy, Electrostatic Force Microscopy, Scanning Capacitance Microscopy.

Tip-enhanced Raman spectroscopy: a combination of the AFM and Raman equipment.

Microelectrodes. Scanning Electrochemical Microscopy.

Examiners

• Kunsági-Máté Sándor

Instructor / tutor of practices and seminars

• Kiss László