Weak Molecular Interactions in Biotechnology

Data

Official data in SubjectManager for the following academic year: 2023-2024

Topic

Micromanipulation in general but particularly in biological systems is mostly based on the modification of chemical equilibria based on reversible weak molecular interactions. Further to this, the weak molecular interactions also play important role in the molecular packing, or molecular formulation of medicines. The weak character of these interactions resulted that both the molecular environment and the temperature has high impact on these interactions. Considering the different temperature dependence of such interactions development within complex structures can be described. Topics from the structure of the solvation shell in binary solutions through development of color of red wines and photo induced polymerization during formation of dental composites or formation complex composites by wide band gap semiconductors and carbon nanostructures will be discussed during the semester. Students will become acquainted through several newest research results with the applications of the processes listed above in Biotechnology and with the associated measuring methods and equipments.

Lectures

• 1. Types of molecular interactions. - Dr. Kunsági-Máté Sándor
• 2. Chemical equilibrium based on the weak molecular interactions. - Dr. Kunsági-Máté Sándor
• 3. An example: Noncovalent Interaction of small molecules with albumins - Dr. Kunsági-Máté Sándor
• 4. Effect of Molecular Environment on the Formation Kinetics of Complexes of Malvidin-3-O-glucoside with Caffeic Acid and Catechin - Dr. Kunsági-Máté Sándor
• 5. Determination of the chemical equilibrium and the stoichiometry of the complexes. - Dr. Kunsági-Máté Sándor
• 6. Role of the Conformational Freedom of the Skeleton in the Complex Formation Ability of Resorcinarene Derivatives toward a Neutral Phenol Guest - Dr. Kunsági-Máté Sándor
• 7. Correlation between the chemical equilibrium and the free enthalpy. - Dr. Kunsági-Máté Sándor
• 8. An example: Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action - Dr. Kunsági-Máté Sándor
• 9. Investigation of the weak interaction by measuring the fluorescence. - Dr. Kunsági-Máté Sándor
• 10. Competitive hydrogen bonds associated with the effect of primycin antibiotic on oleic acid as a building block of plasma membranes - Dr. Kunsági-Máté Sándor
• 11. Application of the Job's method in the fluorescence measurements. - Dr. Kunsági-Máté Sándor
• 12. Complex formation between primycin and ergosterol: entropy-driven initiation of modification of the fungal plasma membrane structure - Dr. Kunsági-Máté Sándor
• 13. Direct measuring the interaction enthalpy by differential scanning calorimetry. - Dr. Kunsági-Máté Sándor
• 14. Thermodynamic and kineTic processes during the unfolding of BSA in the presence of the mycotoxin patulin - Dr. Kunsági-Máté Sándor
• 15. Interactions of aromatic compounds. Interactions of bioactive compounds with DNA. - Dr. Kunsági-Máté Sándor
• 16. Thermodynamic study of the effects of ethanol on the interaction of some toxins with human serum albumin - Dr. Kunsági-Máté Sándor
• 17. Role of the Conformational Freedom of the Skeleton in the Complex Formation Ability of Resorcinarene Derivatives toward a Neutral Phenol Guest - Dr. Kunsági-Máté Sándor
• 18. Investigations of model-systems: interactions of calixarenes with phenols and fullerenes. - Dr. Kunsági-Máté Sándor
• 19. Modifications of structure of serum albumins by molecular capsules. Interactions of molecular capsules with aromatic amino acids at terminated positions. - Dr. Kunsági-Máté Sándor
• 20. Investigations of the conformation changes of proteins by fluorescence and calorimetric methods. - Dr. Kunsági-Máté Sándor
• 21. Antioxidant and antimicrobial properties of randomly methylated beta-cyclodextrin - captured essential oils - Dr. Kunsági-Máté Sándor
• 22. Controlling of self-assembly of some hormones by weak interactions. - Dr. Kunsági-Máté Sándor
• 23. Formulation and detection: Complex formation of flavonoids fisetin and geraldol with beta-cyclodextrins - Dr. Kunsági-Máté Sándor
• 24. Entropy-driven complex formation of malvidin-3-O-glucoside with common polyphenols in ethanol-water binary solutions - Dr. Kunsági-Máté Sándor

Practices

Seminars

Reading material

Obligatory literature

David Freifelder: Physical Biochemistry

Literature developed by the Department

Slides and notes of all lectures are available electronically. All topics discussed will be associated with papers published recently. Those papers together with the related doctoral dissertations in English will also be offered to support the teaching.

Notes

Recommended literature

Atkins: Physical Chemistry I-III.
David Freifelder: Physical Biochemistry

Conditions for acceptance of the semester

Maximum of 15 % absence allowed

Mid-term exams

One written exam during the semester, the acceptance level is 60%.

Making up for missed classes

There is no possibility to make up for a missed lecture and practice

Exam topics/questions

Types of molecular interactions.
Chemical equilibrium based on the weak molecular interactions.
Determination of the chemical equilibrium and the stoichiometry of the complexes.
Correlation between the chemical equilibrium and the Gibbs free energy.
Investigation of the weak interaction by spectroscopical methods.
Application of the Jobs method in the fluorescence measurements.
Direct measuring the interaction enthalpy by differential scanning calorimetry.
Interactions of aromatic compounds.
Interactions of bioactive compounds with DNA.
Investigations of model-systems: interactions of calixarenes with phenols and fullerenes.
Packing of drug molecules by molecular containers.
Interactions of bioactive molecules with elements of cell membranes.
Self-assembly of some hormones by weak interactions.

Examiners

Instructor / tutor of practices and seminars