Electrochemical Sensors in Pharmaceutical and Biomedical Analysis

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

In this teaching, we wish to introduce to the students the main electrodes (specific electrodes, oxygen sensor, glucose biosensor…) and the principles of the electrochemical methods used in the field of pharmaceutical and biomedical analysis. We consider to focus on the enzyme electrochemical biosensors, notably the glucose biosensor, and on the ion selective electrodes, e.g. calcium. These electrodes are used in the blood glucose and in the blood gas detections.

To get deeper insights into the basic processes of several biosensors, thermodynamics and kinetics of weak molecular interactions will be discussed in detail. Examples of host-guest interactions of bioactive guest molecules with cavity-shaped hosts (cyclodextrins, calixarenes, cavitands, cucurbiturils) and their immobilization onto the glassy carbon or platinum electrode surfaces by electro-polymerization will be presented.

In this teaching unit, we will give online asynchronous lectures in order that the students acquire good knowledges in electrochemical sensor uses. Then, we will offer online laboratory sessions (recorded videos of the concepts developed in theoretical classes). Finally, we plan to distribute a project to the students. By mixed group of 4 persons (two students from the University of Rennes 1 and two students from the University of Pécs), the students will collaborate to work on publications of their choice in the field of sensors. The validation of this teaching unit would consist in the delivering of a summary report of the bibliographic work. In this project, the students would be guided by the collaboration of the two teachers (Rennes 1 and Pécs). A guided class directed by the two professors would be offered to explain the objectives of the team project. A final class directed by the two professors would be offered to review and summarize the team contributions.

In 2021-2022, this project would involve the pharmacy students from the Universities of Rennes 1 and Pécs and could be proposed to the other pharmacy students within the EDUC alliance from 2022-2023, and maybe also to the medical students. Students will be known through the most recent scientific results about the pharmaceutical applications of the methods listed above, will have ability for successful application of the related measuring and data-evaluating methods. The curse gives considerable support to earn the drug-designer attitudes of pharmacists.

Lectures

• 1. Basics of electrochemistry, thermodynamic background, Nernst-equation - Abasq-Paofai Marie-Laurence
• 2. Main electrodes (specific electrodes, oxygen sensor, glucose biosensor…) and the principles of the electrochemical methods used in the field of pharmaceutical and biomedical analysis. - Abasq-Paofai Marie-Laurence
• 3. Enzyme-based electrochemical biosensors, notably the glucose biosensor. - Abasq-Paofai Marie-Laurence
• 4. Enzyme-based electrochemical biosensors, notably the glucose biosensor. - Abasq-Paofai Marie-Laurence
• 5. Ion selective electrodes. - Abasq-Paofai Marie-Laurence
• 6. Ion selective electrodes. - Abasq-Paofai Marie-Laurence
• 7. Selective sensing, cavity shape controlled weak interactions of drugs with molecular containers - Kunsági-Máté Sándor
• 8. Thermodynamics and kinetics of weak molecular interactions. - Kunsági-Máté Sándor
• 9. Examples of host-guest interactions of bioactive guest molecules with cavity-shaped hosts (cyclodextrins, calixarenes, cavitands, cucurbiturils). - Kunsági-Máté Sándor
• 10. Examples of host-guest interactions of bioactive guest molecules with cavity-shaped hosts (cyclodextrins, calixarenes, cavitands, cucurbiturils). - Kunsági-Máté Sándor
• 11. Immobolization methods for stabilize the sensor molecules ont he electrode surfaces - Kiss László
• 12. Immobolization methods for stabilize the sensor molecules ont he electrode surfaces - Kiss László

Practices

• 1. Preparation of immobilization layers on glassy carbon and platinum electrodes (video) - Kiss László
• 2. Determination of glucose content in an orange juice solution using an electrochemical biosensor (video) - Abasq-Paofai Marie-Laurence
• 3. Preparation of immobilization layers on glassy carbon and platinum electrodes (video) - Kiss László
• 4. Cyclic voltammetry of vitamin C. (video) - Abasq-Paofai Marie-Laurence

Seminars

• 1. Calculation of thermodynamic parameters of weag interactions between drugs and sensor molecules from spectral data - Kunsági-Máté Sándor
• 2. Calculation of thermodynamic parameters of weag interactions between drugs and sensor molecules from spectral data - Abasq-Paofai Marie-Laurence
• 3. Guided class to explain the team project, student collaborations on distributed projects, feedback class on the team project. - Kunsági-Máté Sándor
• 4. Guided class to explain the team project, student collaborations on distributed projects, feedback class on the team project. - Abasq-Paofai Marie-Laurence

Reading material

Obligatory literature

David Freifelder: Physical Biochemistry

Literature developed by the Department

Slides and notes of all lectures are available electronically. All topics discussed will be associated with papers published recently. Those papers together with the related doctoral dissertations in English will also be offered to support the teaching.

Notes

Slides and notes of all lectures are available electronically. All topics discussed will be associated with papers published recently. Those papers together with the related doctoral dissertations in English will also be offered to support the teaching.

Recommended literature

Slides and notes of all lectures are available electronically. All topics discussed will be associated with papers published recently. Those papers together with the related doctoral dissertations in English will also be offered to support the teaching.

Conditions for acceptance of the semester

Project work in international team.

Mid-term exams

One test, one summary prepared about the topic of lectures and one presentation prepared within the scope of the course.

Making up for missed classes

N/A

Exam topics/questions

Main electrodes (specific electrodes, oxygen sensor, glucose biosensor…) and the principles of the electrochemical methods used in the field of pharmaceutical and biomedical analysis.

Enzyme-based electrochemical biosensors, notably the glucose biosensor.

Ion selective electrodes.

Thermodynamics and kinetics of weak molecular interactions.

Examples of host-guest interactions of bioactive guest molecules with cavity-shaped hosts (cyclodextrins, calixarenes, cavitands, cucurbiturils).

Immobilization onto the glassy carbon or platinum electrode surfaces by electro-polymerization.

Examiners

Instructor / tutor of practices and seminars

• Abasq-Paofai Marie-Laurence
• Kiss László
• Kunsági-Máté Sándor