Data
Official data in SubjectManager for the following academic year: 2024-2025
Course director
-
Sétáló György ifj.
associate professor,
Medical Skills Education and Innovation Centre -
Number of hours/semester
lectures: 42 hours
practices: 0 hours
seminars: 0 hours
total of: 42 hours
Subject data
- Code of subject: OSA-MF1-T
- 3 kredit
- Dentistry
- Basic modul
- autumn
-
Course headcount limitations
min. 5 – max. 100
Topic
To provide a molecular and cell biological basis for anatomy, biochemistry, physiology, pathology, pathophysiology, microbiology and pharmacology studies of dentistry students. The course covers cellular and molecular characteristics of the structure and function of cells, the mechanisms of storage, replication and expression of the genetic information and the methods used to study them.
Lectures
- 1. Orientation - Sétáló György ifj.
- 2. Pro- and eukaryotes - Sétáló György ifj.
- 3. Nucleic acids - Sétáló György ifj.
- 4. Proteins - Sétáló György ifj.
- 5. Light microscopy - Sétáló György ifj.
- 6. Electron microscopy - Berta Gergely
- 7. Restriction endonuleases - Bátor Judit
- 8. DNA cloning, genomic libraries - Sétáló György ifj.
- 9. Polymerase chain reaction - Sétáló György ifj.
- 10. DNA sequencing by Sanger and DNA chips - Bátor Judit
- 11. New generation sequencing - Kemény Ágnes
- 12. Examination of gene expression - Kemény Ágnes
- 13. Transgenic organisms - Kemény Ágnes
- 14. Targeted inhibition of gene expression - Kemény Ágnes
- 15. Immunological methods - Varga Judit
- 16. The cell nucleus - Sétáló György ifj.
- 17. Genome organisation - Sétáló György ifj.
- 18. Structure and chemical composition of chromatin - Sétáló György ifj.
- 19. Phases of the cell cycle - Sétáló György ifj.
- 20. Regulation of the cell cycle - Sétáló György ifj.
- 21. Cell division, mitosis and meiosis - Sétáló György ifj.
- 22. Replication in prokaryotes - Pap Marianna
- 23. Replication in eukaryotes - Pap Marianna
- 24. DNA-repair - Pap Marianna
- 25. Transcription in prokaryotes - Pap Marianna
- 26. Eukaryotic pre-rRNA transcription and processing - Pap Marianna
- 27. Eukaryotic pre-mRNA transcription - Pap Marianna
- 28. Eukaryotic pre-mRNA processing - Pap Marianna
- 29. Components of translation - Pap Marianna
- 30. The mechanism of translation - Pap Marianna
- 31. The genetic code - Pap Marianna
- 32. Gene regulation in prokaryotes - Pap Marianna
- 33. Levels of gene regulation in eukaryotes - Berta Gergely
- 34. Epigenetics - Berta Gergely
- 35. Transcription factors - Berta Gergely
- 36. Rough endoplasmatic reticulum, Golgi-apparatus and protein glycosylation - Bátor Judit
- 37. Endocytosis and vesicular transport - Kemény Ágnes
- 38. Lysosomes and the smooth endoplasmatic reticulum - Sétáló György ifj.
- 39. Reactive oxygen species - Sétáló György ifj.
- 40. Structure and function of mitochondria - Sétáló György ifj.
- 41. Genetic apparatus of mitochondria - Sétáló György ifj.
- 42. Closing lecture - Sétáló György ifj.
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Szeberényi J., Komáromy L.: Molecular Cell Biology Syllabus.
Notes
Recommended literature
Gerald Karp: Cell and Molecular Biology
Cooper, G.M.: The Cell. A Molecular Approach.
Lodish et al.: Molecular Cell Biology
Alberts et al.: Molecular Biology of the Cell
Szeberényi J.: Experiments in Molecular Cell Biology
Conditions for acceptance of the semester
Compliance with the maximum permitted number of absences.
Mid-term exams
Mid-term tests
Making up for missed classes
It is not possible to make up for missed lectures.
Exam topics/questions
1. Main features of prokaryotic and eukaryotic cells
2. Structure and function of nucleotides
3. The DNA molecule
4. DNA as the genetic material (experiments as evidence)
5. Types of RNA
6. Proteins
7. Carbohydrates
8. Lipids
9. Restriction endonucleases
10. Cloning of DNA fragments
11. Genomic libraries
12. Polymerase chain reaction
13. The Sanger DNA sequencing method
14. The Illumina DNA sequencing method
15. Proton sequencing and nanopore sequencing of DNA
16. DNA chips
17. Fluorescence in situ hybridization
18. The Human Genome Project
19. cDNA cloning
20. cDNA libraries
21. Transgenic organisms
22. Targeted gene inactivation at the DNA level
23. Inhibition of endogenous gene expression at the level of mRNA
24. Inhibition of endogenous gene expression at the level of proteins
25. Microscopic detection of specific antigens
26. Immunoprecipitation and Western blotting
27. The structure of the nucleus
28. Unique and repetitive sequences
29. Multilevel chromatin organization
30. The chemical composition of chromatin
31. Main phases of the cell cycle
32. Regulation of the cell cycle
33. Mitosis – how our somatic cells divide
34. Meiosis – how germ cells are generated in humans
35. General features of replication
36. The mechanism of replication in prokaryotes
37. Features of eukaryotic replication
38. Excision repair of DNA
39. Proofreading, Mismatch repair
40. Reparation of double-stranded DNA breaks
41. Prokaryotic RNA synthesis and processing
42. General features of eukaryotic RNA synthesis
43. Synthesis of eukaryotic rRNA
44. Synthesis of pre-mRNA in eukaryotes, modifications at the 5’ and 3’ ends
45. Pre-mRNA splicing and editing
46. Synthesis of aminoacyl-tRNA
47. The structure and function of ribosomes
48. The genetic code
49. Initiation of translation
50. Elongation and termination of translation
51. General features of translation
52. The lactose operon
53. The tryptophan operon
54. Cloning by nuclear transplantation
55. Regulation of pre-mRNA synthesis and processing in eukaryotes
56. Regulation of mRNA transport, translation and degradation in eukaryotes
57. Regulation of protein activity and degradation in eukaryotes
58. Eukaryotic transcription factors
59. The mechanism of action of steroid hormones
60. Rough endoplasmic reticulum
61. Golgi complex and the glycosylation of proteins
62. The mechanism of secretion
63. Endocytosis
64. The mechanism of vesicular transport
65. Lysosomes
66. Smooth endoplasmic reticulum
67. Oxygen free radicals and membrane damage
68. The structure and function of mitochondria
69. The genetic apparatus of mitochondria
70. Mitochondrial diseases
Examiners
- Balogh Bálint
- Bátor Judit
- Berta Gergely
- Bogdán Ágnes
- Boros Melinda
- Brandt Barbara
- Bugyi Beáta
- Csabai-Tanics Tímea Judith
- Gaszler Péter
- Horváth Marianna
- Kemény Ágnes
- Leipoldné Vig Andrea Teréz
- Les Hajnalka
- Németh Marica
- Pap Marianna
- Schipp Renáta
- Sétáló György ifj.
- Stayer-Harci Alexandra
- Szütsné Tóth Mónika Ágnes
- Tarjányi Oktávia
- Varga Judit