Cell Biology - Practice

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

The aim of the course is to provide molecular and cellular biological basic knowledge for future biotechnological studies.

The course covers cellular and molecular characteristics of the structure and functions of the cell. Main topics: functional morphology of eukaryotic cells; mechanisms of cell cycle, replication, DNA repair, transcription, translation, gene regulation and vesicular transport. We will discuss the general structure and function of the cytoskeleton, mitochondria, cell membrane and extracellular matrix components.

Lectures

Practices

• 1. Light microscopy - Pap Marianna
• 2. Light microscopy - Pap Marianna
• 3. Separation techniques; centrifugation/chromatography - Pap Marianna
• 4. Separation techniques; centrifugation/chromatography - Pap Marianna
• 5. Isolation of DNA - Pap Marianna
• 6. Isolation of DNA - Pap Marianna
• 7. Plasmid isolation, electrophoresis - Pap Marianna
• 8. Plasmid isolation, electrophoresis - Pap Marianna
• 9. Restriction endonuclease digestion of human DNA - Pap Marianna
• 10. Restriction endonuclease digestion of human DNA - Pap Marianna
• 11. Isolation of RNA - Pap Marianna
• 12. Isolation of RNA - Pap Marianna
• 13. Lab exam - Pap Marianna
• 14. Lab exam - Pap Marianna

Seminars

Reading material

Obligatory literature

Literature developed by the Department

Notes

Lab manual

Recommended literature

Cooper G.M.: The Cell. A Molecular Approach

Karp G.: Cell Biology

Lodish et al.: Molecular Cell Biology

Alberts et al.: Molecular Biology of the Cell

Conditions for acceptance of the semester

None

Mid-term exams

At the beginning of each practical open-ended questions will be asked about the lab material.

Making up for missed classes

No possibility to make-up the labs.

Exam topics/questions

LABORATORY EXAMINATION QUESTIONS

Theoretical questions

1. Structure and operation of the light microscope

2. Sample preparation for light microscopy

3. Radioactive isotopes in molecular cell biology

4. Homogenization, cell fractionation

5. Hypopycnic and isopycnic gradient centrifugation

6. Gel filtration

7. Ion exchange and affinity chromatography

8. Plasmids, plasmid isolation

9. Nucleic acid electrophoresis

10. Isolation of mammalian DNA

11. Isolation of mammalian RNA

12. Structure and operation of the polarization microscope

13. Structure and operation of the phase-contrast microscope

14. Properties of restriction endonucleases

15. Restriction mapping

Practical questions

16. Observation of prokaryotic cells by immersion objective, Gram staining

17. Determination of cell diameter by light microscope

18. Analysis of human peripheral blood smear, May-Grünwald-Giemsa staining

19. Analysis of light microscopic autoradiographic preparation

20. Analysis of bromodeoxyuridine labeling

21. Preparation of linear density gradient

22. Analysis of the result of gel filtration

23. Analysis of plasmid DNA by agarose gel electrophoresis

24. Analysis of the result of restriction mapping

25. Operation of the photometer - determination of DNA and RNA concentration

26. Operation of the polarization microscope

27. Identification of nuclear components on microscopic pictures

28. Identification of cytoplasmic organelles on microscopic pictures

Examiners

• Balassa Tímea
• Németh Marica
• Pap Marianna

Instructor / tutor of practices and seminars

• Pap Marianna