Daten
Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025
Lehrbeauftragte/r
-
Pap Marianna
associate professor,
Medical Skills Education and Innovation Centre -
Semesterwochenstunden
Vorlesungen: 0
Praktika: 14
Seminare: 0
Insgesamt: 14
Fachangaben
- Kode des Kurses: OBA-107-G
- 1 kredit
- Biotechnology MSc
- Basic modul
- autumn
OBA-107-E parallel
Zahl der Kursteilnehmer für den Kurs:
min. 5 – max. 200
Thematik
The aim of the course is to provide molecular and cellular biological basic knowledge for future biotechnological studies.
The course covers cellular and molecular characteristics of the structure and functions of the cell. Main topics: functional morphology of eukaryotic cells; mechanisms of cell cycle, replication, DNA repair, transcription, translation, gene regulation and vesicular transport. We will discuss the general structure and function of the cytoskeleton, mitochondria, cell membrane and extracellular matrix components.
Vorlesungen
Praktika
- 1. Light microscopy - Pap Marianna
- 2. Light microscopy - Pap Marianna
- 3. Separation techniques; centrifugation/chromatography - Pap Marianna
- 4. Separation techniques; centrifugation/chromatography - Pap Marianna
- 5. Isolation of DNA - Pap Marianna
- 6. Isolation of DNA - Pap Marianna
- 7. Plasmid isolation, electrophoresis - Pap Marianna
- 8. Plasmid isolation, electrophoresis - Pap Marianna
- 9. Restriction endonuclease digestion of human DNA - Pap Marianna
- 10. Restriction endonuclease digestion of human DNA - Pap Marianna
- 11. Isolation of RNA - Pap Marianna
- 12. Isolation of RNA - Pap Marianna
- 13. Lab exam - Pap Marianna
- 14. Lab exam - Pap Marianna
Seminare
Materialien zum Aneignen des Lehrstoffes
Obligatorische Literatur
Vom Institut veröffentlichter Lehrstoff
Skript
Lab manual
Empfohlene Literatur
Cooper G.M.: The Cell. A Molecular Approach
Karp G.: Cell Biology
Lodish et al.: Molecular Cell Biology
Alberts et al.: Molecular Biology of the Cell
Voraussetzung zum Absolvieren des Semesters
None
Semesteranforderungen
At the beginning of each practical open-ended questions will be asked about the lab material.
Möglichkeiten zur Nachholung der Fehlzeiten
No possibility to make-up the labs.
Prüfungsfragen
LABORATORY EXAMINATION QUESTIONS
Theoretical questions
1. Structure and operation of the light microscope
2. Sample preparation for light microscopy
3. Radioactive isotopes in molecular cell biology
4. Homogenization, cell fractionation
5. Hypopycnic and isopycnic gradient centrifugation
6. Gel filtration
7. Ion exchange and affinity chromatography
8. Plasmids, plasmid isolation
9. Nucleic acid electrophoresis
10. Isolation of mammalian DNA
11. Isolation of mammalian RNA
12. Structure and operation of the polarization microscope
13. Structure and operation of the phase-contrast microscope
14. Properties of restriction endonucleases
15. Restriction mapping
Practical questions
16. Observation of prokaryotic cells by immersion objective, Gram staining
17. Determination of cell diameter by light microscope
18. Analysis of human peripheral blood smear, May-Grünwald-Giemsa staining
19. Analysis of light microscopic autoradiographic preparation
20. Analysis of bromodeoxyuridine labeling
21. Preparation of linear density gradient
22. Analysis of the result of gel filtration
23. Analysis of plasmid DNA by agarose gel electrophoresis
24. Analysis of the result of restriction mapping
25. Operation of the photometer - determination of DNA and RNA concentration
26. Operation of the polarization microscope
27. Identification of nuclear components on microscopic pictures
28. Identification of cytoplasmic organelles on microscopic pictures
Prüfer
- Balassa Tímea
- Németh Marica
- Pap Marianna
Praktika, Seminarleiter/innen
- Pap Marianna