Basics in Cell and Tissue Cultures

Data

Official data in SubjectManager for the following academic year: 2026-2027

Topic

The course material discusses the basic principles of cell culture techniques both for cell lines and primary tissues. Fundamental techniques for stem cell purification and stem cell culture will also be detailed. The course will provide both theoretical and practical background for the use of tissue cultures in complex pharmaceutical testing and biotechnological modification of cells and tissues.

Lectures

Practices

• 1. Practicing sterile work in a tissue culture laboratory, thawing up adhesive and non-adhesive cell lines - Papp Alexandra
• 2. Keeping cell lines in culture, passage of cell cultures, counting and pelletting cells, setting up cell cultures at set cell numbers - Papp Alexandra
• 3. Storage and freezing down cell lines. - Papp Alexandra
• 4. Practicing microbial screening I. - Papp Alexandra
• 5. Practicing microbial screening II. - Papp Alexandra
• 6. Testing cell lines for transdifferentiation, apoptosis, etc.. General fingerprinting of cell lines - Papp Alexandra
• 7. Designing immortalization of primary cells - Papp Alexandra
• 8. Purification of primary cells using tissue characteristic markers - Papp Alexandra
• 9. Culturing primary cells and testing survival and apoptotic death - Papp Alexandra
• 10. Purification of adult stem cells based on predicted markers - Papp Alexandra
• 11. Culturing stem cells - Papp Alexandra
• 12. Testing for differentiation markers and cellular proliferation - Papp Alexandra
• 13. Presentation and discussion: Individual experiments designed by students - Papp Alexandra
• 14. Presentation and discussion: Individual experiments designed by students - Papp Alexandra

Seminars

• 1. History of cell cultures and introduction to a cell culture facility - Papp Alexandra
• 2. Cell and tissue types in culture. Origins of cell types, tissue banks - Papp Alexandra
• 3. Regulations on using cell cultures - Papp Alexandra
• 4. Cell culture protocols, risk assessment and microbial screening - Papp Alexandra
• 5. Preparatory techniques used in creating cell lines from complex tissues - Papp Alexandra
• 6. Differentiation and redifferentiation of cell lines. Testing spontaneous transformation. Regular fingerprinting - Papp Alexandra
• 7. Arteficially immortalized cell types - Papp Alexandra
• 8. Experimental designs using cell cultures - Papp Alexandra
• 9. Main characteristics of stem cells and origin of stem cell types - Papp Alexandra
• 10. Stem cell markers and stem cell cultures - Papp Alexandra
• 11. Keeping stem cells in culture - Papp Alexandra
• 12. Directed differentiation of stem cells - Papp Alexandra
• 13. Complex stem cell cultures - Papp Alexandra
• 14. Experimental designs using stem cells - Papp Alexandra

Reading material

Obligatory literature

Literature developed by the Department

ppt

Notes

Basic and Complex cell and Tissue Culture techniques for Medical Biotechnology and Biotechnology MSc Students, PTE, 2014

Recommended literature

Bernice M. Martin: Fundamental Techniques in Cell Culture - a Laboratory Handbook (ECACC?), Tissue Culture Techniques: An Introduction, paperback, Aug 1, 1994

Kerry Atkinson: Clinical Bone Marrow and Blood Stem Cell Transplantation, 3rd edition, Cambridge University Press, 2004

Leda dos Reis Castilho: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Routledge, 2008

Clive Svendsen, Svendsen, Allison D. Ebert: Encyclopedia of Stem Cell Research, Sage eReference, SAGE Publications, 2008

Conditions for acceptance of the semester

Participation on seminars and practices

Mid-term exams

Written exam

Making up for missed classes

None

Exam topics/questions

Neptun MeetStreet, Teams

Examiners

• Pongrácz Judit Erzsébet
• Steinerbrunnerné Nagy Alexandra

Instructor / tutor of practices and seminars

• Draskóczi Lilla Gréta
• Papp Alexandra