Basics in Cell and Tissue Cultures

Adatok

A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2026-2027

Tematika

The course material discusses the basic principles of cell culture techniques both for cell lines and primary tissues. Fundamental techniques for stem cell purification and stem cell culture will also be detailed. The course will provide both theoretical and practical background for the use of tissue cultures in complex pharmaceutical testing and biotechnological modification of cells and tissues.

Előadások

Gyakorlatok

• 1. Practicing sterile work in a tissue culture laboratory, thawing up adhesive and non-adhesive cell lines - Papp Alexandra
• 2. Keeping cell lines in culture, passage of cell cultures, counting and pelletting cells, setting up cell cultures at set cell numbers - Papp Alexandra
• 3. Storage and freezing down cell lines. - Papp Alexandra
• 4. Practicing microbial screening I. - Papp Alexandra
• 5. Practicing microbial screening II. - Papp Alexandra
• 6. Testing cell lines for transdifferentiation, apoptosis, etc.. General fingerprinting of cell lines - Papp Alexandra
• 7. Designing immortalization of primary cells - Papp Alexandra
• 8. Purification of primary cells using tissue characteristic markers - Papp Alexandra
• 9. Culturing primary cells and testing survival and apoptotic death - Papp Alexandra
• 10. Purification of adult stem cells based on predicted markers - Papp Alexandra
• 11. Culturing stem cells - Papp Alexandra
• 12. Testing for differentiation markers and cellular proliferation - Papp Alexandra
• 13. Presentation and discussion: Individual experiments designed by students - Papp Alexandra
• 14. Presentation and discussion: Individual experiments designed by students - Papp Alexandra

Szemináriumok

• 1. History of cell cultures and introduction to a cell culture facility - Papp Alexandra
• 2. Cell and tissue types in culture. Origins of cell types, tissue banks - Papp Alexandra
• 3. Regulations on using cell cultures - Papp Alexandra
• 4. Cell culture protocols, risk assessment and microbial screening - Papp Alexandra
• 5. Preparatory techniques used in creating cell lines from complex tissues - Papp Alexandra
• 6. Differentiation and redifferentiation of cell lines. Testing spontaneous transformation. Regular fingerprinting - Papp Alexandra
• 7. Arteficially immortalized cell types - Papp Alexandra
• 8. Experimental designs using cell cultures - Papp Alexandra
• 9. Main characteristics of stem cells and origin of stem cell types - Papp Alexandra
• 10. Stem cell markers and stem cell cultures - Papp Alexandra
• 11. Keeping stem cells in culture - Papp Alexandra
• 12. Directed differentiation of stem cells - Papp Alexandra
• 13. Complex stem cell cultures - Papp Alexandra
• 14. Experimental designs using stem cells - Papp Alexandra

A tananyag elsajátításához szükséges segédanyagok

Kötelező irodalom

Saját oktatási anyag

ppt

Jegyzet

Basic and Complex cell and Tissue Culture techniques for Medical Biotechnology and Biotechnology MSc Students, PTE, 2014

Ajánlott irodalom

Bernice M. Martin: Fundamental Techniques in Cell Culture - a Laboratory Handbook (ECACC?), Tissue Culture Techniques: An Introduction, paperback, Aug 1, 1994

Kerry Atkinson: Clinical Bone Marrow and Blood Stem Cell Transplantation, 3rd edition, Cambridge University Press, 2004

Leda dos Reis Castilho: Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Routledge, 2008

Clive Svendsen, Svendsen, Allison D. Ebert: Encyclopedia of Stem Cell Research, Sage eReference, SAGE Publications, 2008

A félév elfogadásának feltételei

Participation on seminars and practices

Félévközi ellenőrzések

Written exam

Távolmaradás pótlásának lehetőségei

None

Vizsgakérdések

Neptun MeetStreet, Teams

Vizsgáztatók

• Pongrácz Judit Erzsébet
• Steinerbrunnerné Nagy Alexandra

Gyakorlatok, szemináriumok oktatói

• Draskóczi Lilla Gréta
• Papp Alexandra