Data
Official data in SubjectManager for the following academic year: 2020-2021
Course director
-
Dr. István Miklós ÁBRAHÁM
professor,
Institute of Physiology -
Number of hours/semester
lectures: 12 hours
practices: 0 hours
seminars: 0 hours
total of: 12 hours
Subject data
- Code of subject: OAF-BTP-T
- 1 kredit
- General Medicine
- Optional modul
- autumn
-
Course headcount limitations
min. 5 – max. 25
Available as Campus course for 1 fő számára. Campus-karok: ÁOK GYTK TTK
Topic
The aim of the course is to discuss the common DNA and genome editing techniques focusing on fluorescent protein tagging and bioinformatic tools used in genome and image analysis.
Lectures
- 1. Detection of specific nucleotide sequences - Dr. Kovács Tamás
- 2. Gene activity measurement (qPCR) - Dr. Kovács Tamás
- 3. Online nucleotide databases - Dr. Ernszt Dávid
- 4. Primer design and qPCR analysis - Dr. Ernszt Dávid
- 5. Molecular cloning - Dr. Kovács Tamás
- 6. Viral transduction and transfection techniques - Dr. Kovács Tamás
- 7. Plasmid restriction mapping - Dr. Ernszt Dávid
- 8. In silico analysis - Dr. Ernszt Dávid
- 9. Genome editing techniques - Dr. Kovács Tamás
- 10. CRISPR Cas9 - Dr. Kovács Tamás
- 11. Basics of data analysis - Dr. Ernszt Dávid
- 12. Basics of image analysis - Dr. Ernszt Dávid
Practices
Seminars
Reading material
Obligatory literature
Literature developed by the Department
Topics of the lectures will be available in form of ppt files.
Notes
Recommended literature
Conditions for acceptance of the semester
Maximum of 25 % absence allowed
Mid-term exams
Oral report based on the topics of lectures at the end of the course. Missed oral report can be catch up in that week.
Making up for missed classes
None
Exam topics/questions
Oral report based on the topics of lectures.
Examiners
- Dr. Ábrahám István Miklós
- Dr. Ernszt Dávid
- Dr. Kovács Tamás