Adatok
A Tantárgybejelentőben megadott hivatalos adatok az alábbi tanévre: 2023-2024
Tantárgyfelelős
-
Dr. Beáta BUGYI
associate professor,
Department of Biophysics
Óraszámok/félév
előadás: 0 óra
gyakorlat: 14 óra
szeminárium: 0 óra
összesen: 14 óra
Tárgyadatok
- Kód: OBA-106-G
- 1 kredit
- Biotechnology MSc
- Basic modul
- autumn
OBA-106-E parallel
Vizsgakurzus:Nem
Kurzus létszámkorlát
min. 5 fő
Tematika
The Biophysics curse aims to introduce the students to the methods and applications, which are routinely used in both medical and pharmaceutical biotechnology. The principles of the state-of-the-art approaches and instrumentations are covered by the topics. The course presents diverse spectroscopic techniques (absorption, fluorescence, infrared, Raman, EPR, NMR) imaging approaches (light and fluorescence microscopy, EM, super-resolution fluorescence microscopy, MRI), radioactive applications and calorimetric and fast kinetics techniques. The lectures discuss in detail the physical basis and principles of each approach and the field of applications. The practicals are dedicated to extending the students' knowledge and routine in the use of the different techniques. The practicals lay special emphasis on presenting not only the routine applications but advanced uses of each technique. The limitations, as well as the artifacts that can be caused by improper experimental planning, are highlighted.
Előadások
Gyakorlatok
- 1. Bioinformatic resources
- 2. Bioinformatic resources
- 3. Light microscopy and image analysis
- 4. Light microscopy and image analysis
- 5. Spectroscopy 1
- 6. Spectroscopy 1
- 7. Spectroscopy 2
- 8. Spectroscopy 2
- 9. Multicolor fluorescence, flow cytometry
- 10. Multicolor fluorescence, flow cytometry
- 11. Analytical techniques
- 12. Analytical techniques
- 13. Revision, end semester test
- 14. Revision, end semester test
Szemináriumok
A tananyag elsajátításához szükséges segédanyagok
Kötelező irodalom
Saját oktatási anyag
Homepage of the Department of Biophysics:
https://aok.pte.hu/en/egyseg/10/oktatasi-anyagok
Jegyzet
Ajánlott irodalom
A félév elfogadásának feltételei
Maximum of 15 % absence allowed
Félévközi ellenőrzések
Two written tests are scheduled during the semester covering the topics/calculations/etc discussed during the practices. The expected dates: 7th week, 14th week. The practical grade is obtained based on the average of the two grades of the tests.
Grading policy:
< 60% fail (1)
60 – 69% satisfactory (2)
70 – 79% average (3)
80 – 89% good (4)
> 90% excellent (5)
To pass, at least 60% has to be reached at each test.
Two retake tests will be scheduled at the end of the semester/exam period. The schedule will be provided during the semester.
Távolmaradás pótlásának lehetőségei
The schedule for making up the missed practices will be provided during the semester.
Vizsgakérdések
Topics of the exam questions:
Electromagnetic waves
Quantum numbers, NMR, MRI
Energy levels of atoms and molecules
Laser
UV-VIS absorption spectroscopy
Fluorescence spectroscopy
Infrared and Raman spectroscopy
Rapid kinetic methods
Flow cytometry
Light and fluorescence microscopy
Advanced fluorescence microscopy
Image analysis
Sound, ultrasound
Gamma camera, computed tomography (CT), single-photon emission computed tomography (SPECT), positron emission tomography (PET)
Radioactivity, the interaction of radioactive radiations with matter
Biological effects of radioactive radiations, dosimetry
X-ray diffraction
Thermodynamics: laws and thermodynamic potentials
Calorimetry: differential scanning calorimetry (DSC), isothermal titration calorimetry (ITC)
Analytical separation techniques: sedimentation, electrophoresis
Analytical separation techniques: chromatographic techniques
Mass spectrometry
Vizsgáztatók
Gyakorlatok, szemináriumok oktatói
- Dr. Bugyi Beáta
- Dr. Bukovics Péter
- Dr. Gaszler Péter
- Dr. Szütsné Tóth Mónika Ágnes