Use of the EPR Spectroscopy on Biological Structures

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2020-2021

Thematik

The aim of the course is to learn the usual techniques in EPR spectroscopy on different samples, and evaluation of the results obtained.
The electron paramagnetic resonance spectroscopy is a method similar to NMR, but deals with the properties of electron spin resonance. The technique provides data on the molecular structure and dynamics of various, e.g. biological systems.
The investigation of paramagnetic materials, like free radicals, metals, and special spin-labels help to describe biological systems, e.g. membranes, proteins.

Vorlesungen

• 1. The basics of EPR Spectroscopy - Dr. Dergez Tímea
• 2. The basic features of EPR Spectrometer - Dr. Dergez Tímea
• 3. Spin labels and labeling methods - Dr. Dergez Tímea
• 4. Free radicals - spin trapping - Dr. Dergez Tímea
• 5. Membrane structures and their labeling - Borbásné Dr. Farkas Kornélia
• 6. Other biological structures and their labeling - Borbásné Dr. Farkas Kornélia

Praktika

• 1. Way of registration, registration and analyze of an EPR spectra
• 2. Measurements of membrane dynamics 1.
• 3. Measurements of membrane dynamics 2.
• 4. Labeling and measuring of other biological structures 1.
• 5. Labeling and measuring of other biological structures 2.
• 6. Spin trapping

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

Skript

Empfohlene Literatur

1. C. P. Poole: Electron Spin Resonance, John Wiley & Sons, New York, 1983
2. Hoppe, N., Lohmann, W., Marke, H., Ziegler, H.: Biophysics, Springer-Verlag, Berlin, 1987

Voraussetzung zum Absolvieren des Semesters

Oral exam

Semesteranforderungen

Möglichkeiten zur Nachholung der Fehlzeiten

Consultation

Prüfungsfragen

Prüfer

Praktika, Seminarleiter/innen

• Borbásné Dr. Farkas Kornélia
• Dr. Dergez Tímea