Methods in Molecular Biology and Their Applications in Medical Practice

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2024-2025

Lehrbeauftragte/r

  • Pap Marianna

    associate professor,
    Department of Medical Biology and Central Electron Microscope Laboratory

Semesterwochenstunden

Vorlesungen: 24

Praktika: 0

Seminare: 0

Insgesamt: 24

Fachangaben

  • Kode des Kurses: OBF-085-T
  • 2 kredit
  • Biotechnology MSc
  • Optional modul
  • spring
Voraussetzungen:

keine

Zahl der Kursteilnehmer für den Kurs:

min. 5 – max. 200

Thematik

The aim of the course is to describe interesting and novel molecular biology technologies, including three-dimensional tissue culturing, bioprinting, new generation sequencing techniques, human genetic markers, targeted genome editing. We emphasize the importance and applicability of these methods in research, in the modeling and diagnosis of different diseases.

Vorlesungen

  • 1. Types of cell cultures - Pap Marianna
  • 2. Maintenance and manipulation of cell cultures - Pap Marianna
  • 3. 3D tissue culturing - general features - Pap Marianna
  • 4. 3D tissue culturing - cells and biomaterials - Pap Marianna
  • 5. Bioprinting - general features - Pap Marianna
  • 6. Bioprinting - medical application - Pap Marianna
  • 7. Types of PCR - Pap Marianna
  • 8. PCR - medical application - Pap Marianna
  • 9. Next generation sequencing techniques - Pap Marianna
  • 10. Next generation sequencing techniques - Pap Marianna
  • 11. Human genetic markers - general features - Pap Marianna
  • 12. Methods to study human genetic markers - Pap Marianna
  • 13. Genome editing - sequence-specific nucleases - Pap Marianna
  • 14. Genome editing - CRISPR/Cas9 system and medical application - Pap Marianna
  • 15. Epigenetics - Pap Marianna
  • 16. Methods to study DNA methylation - Pap Marianna
  • 17. Functional genomics - methods to study transcription - Pap Marianna
  • 18. Functional genomics - methods to study translation - Pap Marianna
  • 19. Molecular diagnostics - Pap Marianna
  • 20. Molecular diagnostics - Pap Marianna
  • 21. Types of gene therapy - Pap Marianna
  • 22. Gene therapy - medical application - Pap Marianna
  • 23. Exam - Pap Marianna
  • 24. Exam - Pap Marianna

Praktika

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

Lecture slides

Skript

Empfohlene Literatur

Voraussetzung zum Absolvieren des Semesters

None

Semesteranforderungen

Written test on the last week.

Möglichkeiten zur Nachholung der Fehlzeiten

No possibility to make up the missing lectures.

Prüfungsfragen

1. Types of cell cultures
2. Maintenance and manipulation of cell cultures
3. 3D tissue culturing, Bioprinting
4. Types of PCR, medical application
5. Next generation sequencing techniques
6. Human genetic markers
7. Genome editing
8. Epigenetics
9. Functional genomics
10. Molecular diagnostics
11. Gene therapy, medical application

Prüfer

Praktika, Seminarleiter/innen