Cell Biology - Practice

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2022-2023

Thematik

The aim of the course is to provide molecular and cellular biological basic knowledge for future biotechnological studies.
The course covers cellular and molecular characteristics of the structure and functions of the cell. Main topics: functional morphology of eukaryotic cells; mechanisms of cell cycle, replication, DNA repair, transcription, translation, gene regulation and vesicular transport. We will discuss the general structure and function of the cytoskeleton, mitochondria, cell membrane and extracellular matrix components.

Vorlesungen

Praktika

• 1. Light microscopy
• 2. Light microscopy
• 3. Separation techniques; centrifugation/chromatography
• 4. Separation techniques; centrifugation/chromatography
• 5. Histochemistry of macromolecules
• 6. Isolation of DNA
• 7. Isolation of DNA
• 8. Plasmid isolation, electrophoresis
• 9. Plasmid isolation, electrophoresis
• 10. Plasmid isolation, electrophoresis
• 11. Isolation of RNA
• 12. Isolation of RNA
• 13. Lab exam
• 14. Lab exam

Seminare

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Vom Institut veröffentlichter Lehrstoff

Skript

Lab manual

Empfohlene Literatur

Cooper G.M.: The Cell. A Molecular Approach
Karp G.: Cell Biology
Lodish et al.: Molecular Cell Biology
Alberts et al.: Molecular Biology of the Cell

Voraussetzung zum Absolvieren des Semesters

Maximum of 15 % absence allowed

Semesteranforderungen

At the beginning of each practical open-ended questions will be asked about the lab material.

Möglichkeiten zur Nachholung der Fehlzeiten

No possibility to make-up the labs.

Prüfungsfragen

LABORATORY EXAMINATION QUESTIONS

Theoretical questions

1. Structure and operation of the light microscope
2. Sample preparation for light microscopy
3. Radioactive isotopes in molecular cell biology
4. Homogenization, cell fractionation
5. Hypopycnic and isopycnic gradient centrifugation
6. Gel filtration
7. Ion exchange and affinity chromatography
8. Plasmids, plasmid isolation
9. Nucleic acid electrophoresis
10. Isolation of mammalian DNA
11. Isolation of mammalian RNA
12. Structure and operation of the polarization microscope
13. Structure and operation of the phase-contrast microscope
14. Histochemistry of nucleic acids
15. Histochemistry of the cytoplasm

Practical questions

16. Observation of prokaryotic cells by immersion objective, Gram staining
17. Determination of cell diameter by light microscope
18. Analysis of human peripheral blood smear, May-Grünwald-Giemsa staining
19. Analysis of light microscopic autoradiographic preparation
20. Analysis of bromodeoxyuridine labeling
21. Preparation of linear density gradient
22. Analysis of the result of gel filtration
23. Analysis of plasmid DNA by agarose gel electrophoresis
24. Operation of the photometer - determination of DNA and RNA concentration
25. Operation of the polarization microscope
26. Analysis of nucleic acid histochemistry preparations
27. Analysis of cytoplasm histochemistry preparations
28. Identification of nuclear components on microscopic pictures
29. Identification of cytoplasmic organelles on microscopic pictures

Prüfer

Praktika, Seminarleiter/innen

• Dr. Pap Marianna