Molecular Cell Biology 1

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2025-2026

Thematik

Molecular cell biology aims to introduce the molecular mechanisms of molecular biology and cell biology and to emphasise their importance in medicine. It provides the basis for teaching the different areas of molecular medicine, molecular pathology, molecular diagnostics, pharmacology and gene therapy. The first semester of the course will introduce the molecular biology methods used today, the structure and function of cellular components, the regulation of the cell cycle, the mechanisms of DNA replication and the expression of gene expression.

Vorlesungen

• 1. Educational objectives - Bugyi Beáta
• 2. Comparison of prokaryotic and eukaryotic cells - Bugyi Beáta
• 3. Macromolecules I. Nucleic acids - Bugyi Beáta
• 4. Macromolecules II. Proteins - Bugyi Beáta
• 5. Modern morphological methods I. Light micrsocopy - Bugyi Beáta
• 6. Modern morphological methods II. Electronmicrsocopy - Bugyi Beáta
• 7. Molecular biology methods I. Restriction endonucleases - Sétáló György ifj.
• 8. Molecular biology methods II. DNA cloning. Genomic libraries - Sétáló György ifj.
• 9. Molecular biology methods III. Polymerase chain reaction - Berta Gergely
• 10. Molecular biology methods IV. DNA sequencing: Sanger-sequencing, DNA-chip - Varga Judit
• 11. Molecular biology methods V. DNA sequencing: New generation sequencing - Varga Judit
• 12. Molecular biology methods VI. Analysis of gene expression - Varga Judit
• 13. Molecular biology methods VII. Transgenic organisms - Pap Marianna
• 14. Molecular biology methods VIII. Inhibition of gene expression - Pap Marianna
• 15. Molecular biology methods IX. Immunologioal methods - Pap Marianna
• 16. The cell nucleus - Bátor Judit
• 17. Genome organization - Bátor Judit
• 18. The structure and chemical composition of chromatin - Varga Judit
• 19. Phases of the cell cycle - Sétáló György ifj.
• 20. Regulation of the cell cycle - Sétáló György ifj.
• 21. Cell division. Mitosis, meiosis - Sétáló György ifj.
• 22. DNA replication in prokaryotes - Sétáló György ifj.
• 23. DNA replication in eukaryotes - Sétáló György ifj.
• 24. DNA repair - Sétáló György ifj.
• 25. Transcription I. Transcription in prokaryotes - Pap Marianna
• 26. Transcription II. Synthesis and processing of pre-rRNA in eukaryotes - Pap Marianna
• 27. Transcription III. Synthesis of pre-mRNA in eukaryotes - Pap Marianna
• 28. Transcription IV. pre-m RNA processing in eukaryotes - Pap Marianna
• 29. Translation I. The components of protein synthesis - Bugyi Beáta
• 30. Translation II. The mechanism of protein synthesis - Bugyi Beáta
• 31. Translation III. The genetic code - Bugyi Beáta
• 32. Regulation of gene expression in prokaryotes - Varga Judit
• 33. Regulation of gene expression in eukaryotes I: Levels of gene regulation - Pap Marianna
• 34. Regulation of gene expression in eukaryotes II: Epigenetics - Pap Marianna
• 35. Regulation of gene expression in eukaryotes III: Transcription factors - Pap Marianna
• 36. Rough endoplasmic reticulum. Golgi-aaparatus. Protein glycosylation - Bátor Judit
• 37. Endocytosis. Mechanism of vesicular transport - Bátor Judit
• 38. Cell defense mechanisms I. Lysosomes, smooth endoplasmic reticulum - Berta Gergely
• 39. Cell defense mechanisms II. Biological effict od oxygen free radicals - Berta Gergely
• 40. Mitochondria I. Structure and function - Sétáló György ifj.
• 41. Mitochondria II. Genetic apparatus - Sétáló György ifj.
• 42. Closing lecture. Exam rules - Bugyi Beáta

Praktika

• 1. Light microscopy. Tracing biological molecules inside the cell
• 2. Light microscopy. Tracing of biological molecules inside the cell
• 3. Polarization and phase contrast microscopy
• 4. Polarization and phase contrast microscopy
• 5. Centrifugation, chromatography
• 6. Centrifugation, chromatography
• 7. Plasmid isolation
• 8. Plasmid isolation
• 9. SDS polyacrylamide gel electrophoresis and Westerm blotting
• 10. SDS polyacrylamide gel electrophoresis and Westerm blotting
• 11. Isolation of nucleic acids
• 12. Isolation of nucleic acids

Seminare

• 1. General information
• 2. Biological macromolecules
• 3. Separation methods
• 4. Comparison of pro- and eukaryotic cells
• 5. Molecular biology methods I.
• 6. Molecular biology methods II.
• 7. Molecular biology methods III.
• 8. Molecular biology methods IV.
• 9. Cell nucleus
• 10. Genome organization
• 11. Chromatin
• 12. Cell cycle
• 13. Regulation of cell cycle
• 14. Cell division. Mitosis, meiosis
• 15. DNA replication
• 16. DNA repair
• 17. Transcription in prokaryotes
• 18. Transcription in eukaryotes
• 19. RNA processing
• 20. Translation I.
• 21. Translation II.
• 22. Regulation of gene expression I.
• 23. Regulation of gene expresssion II.
• 24. Regulation of gene expresssion III.
• 25. Rough endoplasmic reticulum. Golgi complex
• 26. Vesicular transport
• 27. Cell defense mechanisms
• 28. Mitochondria
• 29. Final test
• 30. Final test

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

Cooper, G.M.: The Cell. A Molecular Approach.

Vom Institut veröffentlichter Lehrstoff

PotePedia materials

Materials uploaded to the MS Teams lecture group

Test bank questions

Minimum requirement test questions

Skript

Molecular Cell Biology Laboratory Manual

Szeberényi J., M. Pap (editor): Molecular Cell Biology Syllabus

Empfohlene Literatur

Lodish et al.: Molecular Cell Biology

Alberts et al.: Molecular Biology of the Cell

Voraussetzung zum Absolvieren des Semesters

Completion of all practices, confirmed by the instructor's signature. No more absences from seminars and practices than allowed. According to the application of the provision of Section 1/A (6) of the Code of Studies and Examinations to small group sessions (seminars, practices).

Semesteranforderungen

Mid-term and end-of-semester written tests. The dates and topics of the tests will be announced during the lecture and in the Molecular Cell Biology lecture Teams group. There is no possibility for retakes for any of the written tests. Based on the combined results of the mid-term tests, exemption from the exam can be obtained.

Möglichkeiten zur Nachholung der Fehlzeiten

Due to absences, make-up practicals can be attended at make-up sessions organized by the Department.

Mid-term tests can be made up according to the options designated by the course leader. There is no possibility to make up the end-of-semester test.

Prüfungsfragen

Available on PotePedia and uploaded to the MS Teams lecture group.

Prüfer

• Atlaszné Váczy Alexandra
• Balassa Tímea
• Balogh Bálint
• Bátor Judit
• Berta Gergely
• Bogdán Ágnes
• Boros Melinda
• Bugyi Beáta
• Csabai-Tanics Tímea Judith
• Feketéné Kiss Katalin
• Gaszler Péter
• Horváth Marianna
• Leipoldné Vig Andrea Teréz
• Németh Marica
• Pap Marianna
• Rátky Fanni
• Schipp Renáta
• Sétáló György ifj.
• Stayer-Harci Alexandra
• Tarjányi Oktávia
• Varga Judit

Praktika, Seminarleiter/innen

• KURZUSHOZ RENDELT OKTATÓ