Introduction to Genetic Engineering

Daten

Offizielle Daten in der Fachveröffentlichung für das folgende akademische Jahr: 2023-2024

Lehrbeauftragte/r

Semesterwochenstunden

Vorlesungen: 0

Praktika: 0

Seminare: 22

Insgesamt: 22

Fachangaben

  • Kode des Kurses: OXF-BGS-h-T
  • 2 kredit
  • General Medicine
  • Optional modul
  • spring
Voraussetzungen:

keine

Zahl der Kursteilnehmer für den Kurs:

min. 5 – max. 20

Erreichbar als Campus-Kurs für 10 fő számára. Campus-karok: ÁOK TTK

Thematik

The aim of the course is to introduce students to the methods of manipulating genetic material (genetic engineering). It presents the basic techniques and latest possibilities of genetic engineering, as well as its role in research, drug development and medicine. During the course, students have to design a small genetic engineering project, and the acceptance of the semester and the final grade depends on the completed project. The project must be prepared with the help of a small computer program that simulates genetic engineering and runs under the Windows operating system. Therefore, in order to successfully complete the course, the student must be able to run the program either on a computer with Windows operating system or on an Apple machine in emulation mode.

Vorlesungen

Praktika

Seminare

  • 1. Structure and function of nucleic acids
  • 2. Modified and artificial nucleic acids
  • 3. Oligonucleotide and gene synthesis
  • 4. Gel electrophoresis and sequencing
  • 5. Polymerases
  • 6. Polymerase chain reaction
  • 7. Enzymes to manipulate nucleic acids I.
  • 8. Enzymes to manipulate nucleic acids II.
  • 9. cDNA cloning, basic cloning vectors
  • 10. Expression vectors
  • 11. Transformation, transfection
  • 12. Viral vectors, transposone systems
  • 13. Transgenic and knockout animals
  • 14. Lox-P system, conditional KO animals
  • 15. RNA interference, knockdown techniques
  • 16. Artificial nucleases, genome engineering
  • 17. Optogenetics
  • 18. Artificial evolution techniques
  • 19. Gene therapy
  • 20. Genetic engineering in drug discovery
  • 21. Project evaluation I
  • 22. Project evaluation II

Materialien zum Aneignen des Lehrstoffes

Obligatorische Literatur

None

Vom Institut veröffentlichter Lehrstoff

Power point slides

Skript

None

Empfohlene Literatur

None

Voraussetzung zum Absolvieren des Semesters

None

Semesteranforderungen

The genetic engineering project must be prepared during the semester and submitted before the start of the exam period.

Möglichkeiten zur Nachholung der Fehlzeiten

None

Prüfungsfragen

None

Prüfer

Praktika, Seminarleiter/innen

  • Dr. Sándor Zoltán