Advanced Genetics

Data

Official data in SubjectManager for the following academic year: 2024-2025

Topic

Students completing the course will know the up-to-date developments of the concepts of the genetics field and biotechnology-related genetic applications. Students entering the Biotechnology MSc program are already able to interpret and explain some aspects of the nature of biological variability and genetic processes at a BSc level. The basics of genetics will be briefly repeated with students having different background knowledge. Students will learn about the theoretical background of advanced molecular methods and approaches in the field. A broad methodological spectrum applied in clinical and basic research will be discussed. Lectures will give an up-to-date insight into state-of-the-art applications of genetics, including high throughput technologies, cancer genomics, as well as basic approaches for the analysis of the generated genomic data.

Lectures presented by experts in the field will address relevant problems and challenges of current and future biotechnology. Students will gather a deep understanding and a complex view of modern genetics.

Lectures

• 1. Introduction: a brief overview of the knowledge gained at the BScExpectations, recommended literature - Juhász Kata
• 2. Sequencing methods, genome sequencing - Berenténé Bene Judit Ágnes
• 3. NGS and novel sequencing platforms - Urbán Péter
• 4. Genome editing, CRISPR - Gombos Katalin
• 5. Transcriptomics and functional genomics - Gombos Katalin
• 6. Extranuclear genomes. Transposons - Juhász Kata
• 7. Epigenetics - Rauch Tibor Attila
• 8. Interim test - Juhász Kata
• 9. Cancer genomics - Juhász Kata
• 10. Data analysis for genomics - Gyenesei Attila
• 11. Population genomics. HAPMAP - Gombos Katalin
• 12. Clinical applications of genomic testing, PGD, NiPT - Gombos Katalin
• 13. Methods of gene therapy - Juhász Kata
• 14. Future Genetics - Gallyas Ferenc

Practices

• 1. Introduction - Juhász Kata
• 2. Introduction - Juhász Kata
• 3. Digestions of target and source vectors - Juhász Kata
• 4. Digestions of target and source vectors - Juhász Kata
• 5. Agarose gelelectrophoresis; excision of fragments and digested vectors - Juhász Kata
• 6. Agarose gelelectrophoresis; excision of fragments and digested vectors - Juhász Kata
• 7. Fragment isolation; concentration measurements by Nanodrop; Agar plate preparation - Juhász Kata
• 8. Fragment isolation; concentration measurements by Nanodrop; Agar plate preparation - Juhász Kata
• 9. Ligation; Bacteria transformation and plating - Juhász Kata
• 10. Ligation; Bacteria transformation and plating - Juhász Kata
• 11. Colony PCR reaction - Juhász Kata
• 12. Colony PCR reaction - Juhász Kata
• 13. Agarose gelelectrophoresis; Introduction to culturing bacteria - Juhász Kata
• 14. Agarose gelelectrophoresis; Introduction to culturing bacteria - Juhász Kata
• 15. Plasmid preparation; concentration measurement by Nanodrop; agarose gelelectrophoresis - Juhász Kata
• 16. Plasmid preparation; concentration measurement by Nanodrop; agarose gelelectrophoresis - Juhász Kata
• 17. Transfection of cancer cell lines by nucleofection - Juhász Kata
• 18. Transfection of cancer cell lines by nucleofection - Juhász Kata
• 19. Preparation of protein lysates from transfected cell for subsequent Western blot - Juhász Kata
• 20. Preparation of protein lysates from transfected cell for subsequent Western blot - Juhász Kata
• 21. Fluorescence microscopy of transfected cells - Juhász Kata
• 22. Fluorescence microscopy of transfected cells - Juhász Kata
• 23. Sequencing - Juhász Kata
• 24. Sequencing - Juhász Kata
• 25. Sequence analysis - Juhász Kata
• 26. Sequence analysis - Juhász Kata
• 27. Consultation - Juhász Kata
• 28. Consultation - Juhász Kata

Seminars

Reading material

Obligatory literature

No

Literature developed by the Department

Literature developed by the Department. Powerpoint material includes the basic concept of the subject and gives a reasonable basis for the studies. They can be used with notes prepared by the students in the lectures. Presentations are concise and have a high explanatory force.

Notes

No

Recommended literature

• Leon E. Rosenberg, Diane Drobnis Rosenberg (2013) Genes and Genomes, Science, Health, Society, available online (Elsevier ScienceDirect)

• Muhammad Sarwar Khan, Iqrar Ahmad Khan, Debmalya Barh: Applied Molecular Biotechnology (2016) CRC Press

Conditions for acceptance of the semester

-

Mid-term exams

An interim test containing 10-15 multiple choice test questions and 2 essay questions on the 8th educational week. In the case of reaching or surpassing 90% on the test, a 10% improvement will be added to the final written exam score.

Making up for missed classes

Individual tutoring

Exam topics/questions

Written examination, tests contain 10-15 multiple-choice test questions and 5 essay questions from the topics discussed during the lectures.

Examiners

• Gombos Katalin
• Juhász Kata

Instructor / tutor of practices and seminars

• Berenténé Bene Judit Ágnes
• Gallyas Ferenc
• Gombos Katalin
• Gyenesei Attila
• Juhász Kata
• Rauch Tibor Attila
• Urbán Péter